LOCUS       sequence-316181-        9146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-sfGFP-GSAx9-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..9146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1745..1747,1748..2458)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1745..2458
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1775..1803)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2398..2418
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3575..4510
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3623..4516
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    4553..5141
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4606..4626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5024..5035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5180..5197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5186..5410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5764..5798
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5885..5904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5886..5904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5911..5928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5911..5927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5920..5942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6069..6524)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6206..6227)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6418..6437
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6551..6655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6656..6724,6725..7516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6874..6893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7687..8275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8176..8195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8429..8446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8563..8584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8599..8604,8605..8622,8623..8629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8637..8653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8642..8664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8661..8677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8661..8677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8696..8716
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8698..8716
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8780..8842
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crgggggggg ggggsggggs grggggsggg
      541 gsggggsgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggrg ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg ctagcgccgc
     1741 caccatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1801 tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa
     1861 cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac
     1921 acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa
     1981 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
     2041 tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct
     2101 tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca
     2161 caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa
     2221 tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc
     2281 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2341 ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt
     2401 ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg
     2461 aagcgctggc tccgccgcag ggtctgggag tgccggctcc gctgcaggaa gcggatctgc
     2521 cggcagcgca gctgggtcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt
     2581 ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc
     2641 cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg
     2701 caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct
     2761 gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa
     2821 catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt
     2881 gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc
     2941 ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg acagatgcca
     3001 ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga
     3061 aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca
     3121 cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg
     3181 ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa
     3241 ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact
     3301 gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa
     3361 ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc
     3421 cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac
     3481 caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg gggccatggt
     3541 gaggctgctc gaggatgggg acctcgagcc atctgctgga gacatgagag ctgccaacct
     3601 ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac
     3661 ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta
     3721 tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga
     3781 cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac
     3841 cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct
     3901 cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga
     3961 caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac
     4021 atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat
     4081 tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga
     4141 gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc
     4201 caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct
     4261 ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa
     4321 gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc
     4381 gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc
     4441 gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt
     4501 ccctgccaca gcttgattaa ttaatgatca tacagtgctc tgagtacgct ataatcaacc
     4561 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4621 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4681 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4741 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4801 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4861 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4921 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4981 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5041 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5101 ccctcagacg agtcggatct ccctttgggc cgcctccccg caggatccac tagagcggcc
     5161 gcctagagct cgctatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5221 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5281 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5341 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5401 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc ttgcggaacc
     5461 cttaagcttt tttattatat attagtcacg atatctataa caagaaaata tatatataat
     5521 aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta
     5581 aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa
     5641 tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga
     5701 tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga
     5761 atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt tgcgctttac
     5821 tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac attgtgttgg
     5881 gtcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     5941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6061 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6121 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6181 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6241 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6301 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6361 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6421 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6481 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7561 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8341 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8401 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8461 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8521 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8581 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8641 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8701 taaccctcac taaagggaac ctcccccggt attcacgaca gcaggctgaa taataaaaaa
     8761 attagaaact attatttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
     8821 tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
     8881 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
     8941 caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata
     9001 aagtaacaaa acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa
     9061 aaacagtcat gttgtattat aaaataagta attagcttaa cctatacata atagaaacaa
     9121 attatactta ttaaccggtc atggtc
//
