LOCUS       sequence-31630-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Kim JS, Bonifant C, Bunz F, Lane WS, Waldman T
  TITLE     Epitope tagging of endogenous genes in diverse human cell lines.
  JOURNAL   Nucleic Acids Res. 2008 Nov . 36(19):e127.
  PUBMED    18784188
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2008 Nov . 36(19):e127."
COMMENT     Sequence Label: pAAV-SEPT-Acceptor
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          33..262
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     245..264
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    391..853
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(447..464)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     674..693
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(920..939)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
       61 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
      121 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
      181 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
      241 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcgtcga
      301 gaacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      361 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      421 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      481 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      541 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      601 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      661 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      721 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      781 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      841 aacacgatga taagcttgcc acaaccatgg ctgaacaaga tggattgcac gcnnttctcc
      901 ggccgcttgg gtggagaggc tattcggcta tgactgggca c
//
