LOCUS       sequence-316580-        9477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018341
FEATURES             Location/Qualifiers
     source          1..9477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2181..2201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2202..2225
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2251..2307
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2308..2325)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2308..2324)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2317..2339)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2533..2552)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2652..2674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2712..2730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3516..4715
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4946..6094)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6366..6737)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6770..6879
                     /note="incP origin of transfer"
     rep_origin      7032..7663
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8126..8714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8615..8634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8868..8885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9002..9023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9038..9043,9044..9061,9062..9068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9076..9092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9081..9103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9100..9116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9100..9116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgtcgc
      361 tgaagcgcgc cactggccag accggcctcc agaagaagcg cctttcgcgc gcggtggcga
      421 ccgccatcgc cgggtttgcc ctggcctcag cgtcgggcgc tgccctcgcc aacgccgagc
      481 tgatcaagct gtcgcaggac gatcgccaat gggtcatgca gggcaaggac tatgaccact
      541 cgcactacag cggtctgaag cagatcaatc ggtcgaacgt caagaacctc aaggcggcct
      601 ggaccttctc caccggcgtc ctccacggcc atgagggcgg cccgttggtg gtgaacggca
      661 tcatgtacat ccatacgccc ttcccgaaca tcacgtacgc catcgatctc gataacccca
      721 acaagatcct ctgggagcac aagccaaagc aggatgccag cgtccgggcc gtggcctgct
      781 gcgacgtggt taaccgcggc ctggcgtacg gcgacggtaa gatcttcaag acccagctcg
      841 acggccacat cgtcgctctc gatgctaaga cggggaagga gctctggaag atggagaact
      901 cggaccccaa ggtgggcagc acgctgactc aggcgccgat ggttgtcgag gacatggtga
      961 tcgtggggtc cagtggcgcg gagctcggcg tccgcgggta tgtcaccgcg taccgaatca
     1021 aggacggcaa gcaggtgtgg agggcctacg ccacggggcc cgacgaagac atactgctgg
     1081 cggacgattt caacatggcc aaccctcact acggacagaa gggcctgggc acatctacgt
     1141 gggaggggga cgcctggaag atcggcggcg gcaccaattg gggctggtat gcctacgatc
     1201 cggagcttaa gctgatgtac tatggcagcg gcaaccccgc cccctggaac gagaccatgc
     1261 ggccgggcga caacaagtgg accatggcta tctggggccg tgacgtcaag acgggcaagg
     1321 cgaagttcgc cttccagaag acgccccacg acgagtggga ttacgccggg gtcaattgga
     1381 tcggactgtc cgaccaggtc gtcgatggca agaagcagaa gctcctgacc catccagacc
     1441 gcaatggcat cgtgtacacc ctgaatcgcg aaaacggcaa cctcgtgcgc gccgacaaaa
     1501 tcgatccgtc ggtgaacgtg tttaagggct acgatatgaa gaagggcgtc ccgatccgcg
     1561 accctgagta ttccacccgt atggatcacc tcgccaaggg catctgcccc tcagccatgg
     1621 ggtaccacaa ccagggacac gattctatcg acaagaagcg cgagctggtc atgctcggga
     1681 caaacatgat ctgcatggac tgggagccct tcatgctccc ctaccgggcg gggcagttct
     1741 ttgtgggcgc gacgctgaat atgtacgcga ccccgggcaa caacggtaac atggggcagg
     1801 tgaaggccta tgacgtgaag acgagcaagt tcaagtgggt caaggacgaa aagttctccg
     1861 tgtggggcgg caccacctcc accgctggcg acctggtctt ctacggcacc ctcgacgggc
     1921 tgatcaaggc cgtggacgcc gacaatggga agaccttgtg ggagttcaag ctcccctcgg
     1981 gcgtcatcgg ccatcccgtc acatacgagc acaaaggcaa gcagtacgtc gcgatctatt
     2041 acggtgtcgg gggctggccg ggcgtcggcc tggtcttcga cctcaccgat cccaccgcgg
     2101 gtctgggcgc cgtgggggcg ttcaaggagc tgcagcagta cacgaagatg ggcggtggcg
     2161 tgatggtgtt ctccctcgcc gaaaacctct attttcaggg acatcatcac catcaccatc
     2221 accactgaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga
     2281 ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
     2341 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2401 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2461 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     2521 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2581 aacacccgct gacgcgccct gacgggcttg tatgctcccg gcatccgctt acagacaagc
     2641 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2701 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2761 ttcttagcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
     2821 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
     2881 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
     2941 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
     3001 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
     3061 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
     3121 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
     3181 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
     3241 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
     3301 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
     3361 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
     3421 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
     3481 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg
     3541 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
     3601 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
     3661 tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
     3721 gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccactgtc gactacgcca
     3781 tcatggcgac agcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca
     3841 ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc
     3901 gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg
     3961 tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct
     4021 tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac
     4081 gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca
     4141 tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc
     4201 cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg
     4261 gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc
     4321 ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca
     4381 caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc
     4441 tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg
     4501 atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga
     4561 tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt
     4621 gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc
     4681 tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca
     4741 tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta gaattgtcaa ttttaatcct
     4801 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     4861 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     4921 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     4981 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     5041 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     5101 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     5161 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     5221 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     5281 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     5341 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     5401 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     5461 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     5521 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     5581 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     5641 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     5701 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     5761 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     5821 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     5881 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     5941 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     6001 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     6061 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     6121 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     6181 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     6241 acttggtatt ccgaatcttg ccctgcacga ataccagctc cgcgaagtcg ctcttcttga
     6301 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
     6361 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
     6421 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
     6481 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
     6541 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
     6601 ccgacggcca gcaggtaggc ctacaggctc atgccggccg ccgccgcctt ttcctcaatc
     6661 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
     6721 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
     6781 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     6841 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
     6901 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     6961 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt
     7021 cgaccctttc cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     7081 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
     7141 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     7201 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     7261 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     7321 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     7381 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     7441 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     7501 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     7561 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     7621 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     7681 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagccaagct
     7741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8881 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8941 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9001 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9061 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9121 attacgccaa gctagcttcc cgcttggtcg ggccgcttcg cgagggcccg ttgacgacaa
     9181 cggtgcgatg ggtcccggcc ccggtcaaga cgatgccaat acgttgcgac actacgcctt
     9241 ggcactttta gaattgcctt atcgtcctga taagaaatgt ccgaccagct aaagacatcg
     9301 cgtccaatca aagcctagaa aatataggcg aagggacgct aataagtctt tcataagacc
     9361 gcgcaaatct aaaaatatcc ttagattcac gatgcggcac ttcggatgac ttccgagcga
     9421 gcctggaacc tcagaaaaac gtctgagaga taccgcggat ctcacacagg aaatttc
//
