LOCUS       sequence-31659-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM
  TITLE     Engineered allosteric activation of kinases in living cells.
  JOURNAL   Nat Biotechnol. 2010 Jul;28(7):743-7. doi: 10.1038/nbt.1639. Epub 
            2010 Jun 27.
  PUBMED    20581846
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1639"; 
            journalName: "Nat Biotechnol"; date: "2010-07"; volume: "28"; issue:
            "7"; pages: "743-7"
COMMENT     Sequence Label: pCherry-FRB
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             293..571
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    746..867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(783..802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     837..856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtccgga ctcagatctc gagctcaagc ttcgaattca atggcttcta gaatcctctg
      301 gcatgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa
      361 cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac ggggccccca
      421 gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg aggcccaaga
      481 gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcctccaag cctgggacct
      541 ctattatcat gtgttccgac gaatctcaaa gactagttaa ccgtacgacg taccagacta
      601 cgcataagaa aagtgaggat ccaccggatc tagataactg atcataatca gccataccac
      661 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      721 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      841 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
      901 aattcgcgtt aaa
//
