LOCUS       sequence-31660-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM
  TITLE     Engineered allosteric activation of kinases in living cells.
  JOURNAL   Nat Biotechnol. 2010 Jul;28(7):743-7. doi: 10.1038/nbt.1639. Epub 
            2010 Jun 27.
  PUBMED    20581846
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1639"; 
            journalName: "Nat Biotechnol"; date: "2010-07"; volume: "28"; issue:
            "7"; pages: "743-7"
COMMENT     Sequence Label: pEGFP-FRB
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..345
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    520..641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(557..576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     611..630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(785..806)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttcaatggct
       61 tctagaatcc tctggcatga gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac
      121 tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg
      181 gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta
      241 atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcctc
      301 caagcctggg acctctatta tcatgtgttc cgacgaatct caaagactag ttaaccgtac
      361 gacgtaccag actacgcata agaaaagtga ggatccaccg gatctagata actgatcata
      421 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      481 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      601 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
      661 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      721 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      781 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      841 aaaaaccgtc tatc
//
