LOCUS       sequence-316635-        9504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018355
FEATURES             Location/Qualifiers
     source          1..9504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2208..2228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2229..2252
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2278..2334
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2335..2352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2335..2351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2344..2366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2560..2579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2679..2701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2739..2757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3543..4742
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4973..6121)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6393..6764)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6797..6906
                     /note="incP origin of transfer"
     rep_origin      7059..7690
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8153..8741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8642..8661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8895..8912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9029..9050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9065..9070,9071..9088,9089..9095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9108..9130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9127..9143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9127..9143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaaga
      361 agccggtgaa gaactggcta ctggcctcga ccgtggccac cctcctcgcc gccccgaccg
      421 tgtccacggc caactcggac gtggaaaaac tgacccagga ccccgccaac tgggccacct
      481 ggggcggaga ctacgccggc acccgctact ccaagctcag ccagatcaac gcccagaacg
      541 tcaagaatct gcagccggcg tggtcgttca gcacaggggt cctccggggg cacgagggcg
      601 gccccctcgt ggtcaacgga gtcatgttca tccatacccc gttccccaac accgtctacg
      661 cgatcgacca gaagacgaag gcagtcatct gggagtttac gccgaccatg gacgccgacg
      721 ttaccatccc ggtcatgtgc tgcgacaccg tcaaccgcgg tctggcctac ggtgacggga
      781 agattttcct tcagcagtcc gacacggtgc ttacagccct cgacgccaag acgggcaaac
      841 gcgtgtggtc ggtgcagaat ggcgacccca agctggggat gacaaatacc aatgcccccc
      901 tggtcgtcaa ggataaggtg atcacgggca tctcgggcgg cgagttcggc gtgcgcggct
      961 tcctggccgc ctacaacatc cggaccggcc agctggaatg gaaggggtac tcgatgggcc
     1021 ccgacaagga cacgctgctg aagccgggta agagcaccac ctggcaggat ggtaaggtga
     1081 cgccggtggg cgccgattcc ggcacgtcca cgtggaaggg cgaccagtgg aagatcggtg
     1141 gcgggacgac ctggggctgg tacagctacg atccgaagct caacctcgtt tactacggct
     1201 ccggtaaccc gagcacctgg aacccggtcc agcggccggg cgacaacaag tggtcgatgt
     1261 ctctctgggc ccgggacgcc gacaccggag aggtgaagtg ggtgtatcag atgacaccgc
     1321 acgatgaatg ggactacgat ggcatcaacg agaccgtcct ggttgaccag gaggtgaagg
     1381 gcaagatgca taagaccatc gtgcacttcg acaggaacgg cttcggctac acgctcgacc
     1441 gggagacggg ggaacttctc gtcgccgaaa agttcgacaa gtcggtcaac tgggcctccc
     1501 acgtcgatct gaagtcgggc cggccggagg tcgtgccgga attctccacg gagcacaacg
     1561 gcgaggatgt gaacacggtc ggcacgtgcc ctgcggccct cggctcgaag aaccagcagc
     1621 ccgtctccta ctcgcctcag accggcctgt tctatatctc ggggaaccac ctctgcatgg
     1681 agtacgagcc gttcgaggtc tcctacaccg ccgggcaacc gtacgtgggc gccaccctgt
     1741 ccatgatgcc cgcgggcgcg gacgtcctca ccggcaagaa ggacggcacc accaacctcg
     1801 gccagttcac ggcgtacgat gcgaagacgg gcaagatcgc ttggagcaac aaggagcaat
     1861 tctcggtctg gtcgggctcg gtggcgactg cgggcggcgt cgtcttttac ggcaccctgg
     1921 aaggctacct gaaggccgtc gacgcgaaaa ccggcaagga gctctacaag ttcaaaaccc
     1981 cgtcgggcat catcgggaac gtgaacacct gggaattcga gggcaagcaa tacgtgggcg
     2041 tcctctcggg catcggcggc tgggctggca tcgggatcgc ggccggcatc gacgacggga
     2101 cctccgcgtc gtcctccgag gggctgggcg cggtgggcgc ctaccgcagc ctctcgagct
     2161 acaccaagct gggaggcacg ctgaccgtgt tcgcgctccc gaacgccgaa aacctctatt
     2221 ttcagggaca tcatcaccat caccatcacc actgacagct atgaccatga ttacgccaag
     2281 cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga attcactggc
     2341 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2401 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2461 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2581 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtat
     2641 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2701 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     2761 ataggttaat gtcatgataa taatggtttc ttagcaccct ttctcggtcc ttcaacgttc
     2821 ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac
     2881 gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc
     2941 ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca
     3001 agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc
     3061 gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg
     3121 cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg
     3181 aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa
     3241 tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc
     3301 ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc
     3361 gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc
     3421 ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact
     3481 tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag
     3541 acgtgaaacc caacataccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg
     3601 gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg
     3661 acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttggtgcaa tttgcctgcg
     3721 cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccaatcttg ctcgtctcgc
     3781 tggccggcgc cactgtcgac tacgccatca tggcgacagc gcctttcctt tgggttctct
     3841 atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata
     3901 ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt
     3961 tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc
     4021 acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc
     4081 ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc
     4141 tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct
     4201 tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc
     4261 gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt
     4321 cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac
     4381 tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg
     4441 gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc
     4501 tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac
     4561 tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg
     4621 cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc
     4681 tctgcctgcc ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct
     4741 gatcgtggaa acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg
     4801 cgccctagaa ttgtcaattt taatcctctg tttatcggca gttcgtagag cgcgccgtgc
     4861 gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg
     4921 ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt
     4981 tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact
     5041 cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc
     5101 acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt
     5161 cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt
     5221 agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg
     5281 ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc
     5341 caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg
     5401 ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg
     5461 tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca
     5521 ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt
     5581 tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg
     5641 tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg
     5701 caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg
     5761 cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct
     5821 tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct
     5881 gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca
     5941 gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact
     6001 ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg
     6061 cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca
     6121 ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat
     6181 ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc
     6241 cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata
     6301 ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc
     6361 gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg gcggaccacg
     6421 cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg
     6481 atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg
     6541 ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg ctcatagtcc
     6601 acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccta caggctcatg
     6661 ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg
     6721 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct
     6781 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     6841 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     6901 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     6961 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     7021 cagggttatg cagcggaaaa gatccgtcga ccctttccga cgctcaccgg gctggttgcc
     7081 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
     7141 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     7201 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     7261 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     7321 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     7381 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     7441 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     7501 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     7561 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     7621 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     7681 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
     7741 cctcacccca aaaatggcag ccaagctgac cacttctgcg ctcggccctt ccggctggct
     7801 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7861 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7921 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7981 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8281 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8761 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8821 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8881 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8941 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9001 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9061 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9121 ttcacacagg aaacagctat gaccatgatt acgccaagct agcttcccgc ttggtcgggc
     9181 cgcttcgcga gggcccgttg acgacaacgg tgcgatgggt cccggccccg gtcaagacga
     9241 tgccaatacg ttgcgacact acgccttggc acttttagaa ttgccttatc gtcctgataa
     9301 gaaatgtccg accagctaaa gacatcgcgt ccaatcaaag cctagaaaat ataggcgaag
     9361 ggacgctaat aagtctttca taagaccgcg caaatctaaa aatatcctta gattcacgat
     9421 gcggcacttc ggatgacttc cgagcgagcc tggaacctca gaaaaacgtc tgagagatac
     9481 cgcggatctc acacaggaaa tttc
//
