LOCUS       sequence-316722-        7685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Nup93 fused at its carboxyl-terminus to 
            mEGFP with no linker and a total net fusion of (-5 amino acids) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7685)
  TITLE     Simon lab plasmids
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Nup93_mEGFP(-5)
FEATURES             Location/Qualifiers
     source          1..7685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(286..305)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    330..377
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      706..1294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1195..1214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1469..1772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1773..1976
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1927..1947
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4571..4592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4832..4851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5179..5200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5881..6002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5918..5937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5972..5991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6009..6464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6358..6377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6491..6595
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6597..6954
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6597..6788
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6620..6640)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6805..6940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(6989..7685,1..98)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(6989..7685,1..98)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7043..7062)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7653..7672
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
       61 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
      121 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
      181 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
      241 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
      301 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
      361 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
      421 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
      481 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
      541 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
      601 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
      661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1381 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     1441 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1501 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1561 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1621 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1681 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1741 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1801 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1861 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1921 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     1981 tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atgtccggac tcagatctcg
     2041 acttcccggg atgacggctg tagcacaagg atctgcatct ccaatggata ctgaggggtt
     2101 tggtgagctc cttcagcaag ctgaacagct tgctgctgag actgagggca tctcagagct
     2161 tccccatgtg gaacggaact tacaggagat ccagcaggcg ggagagcgcc tgcgttcccg
     2221 taccctaaca cgcacgtccc aggagacggc agatgtcaag gcgtcagttc tcctcgggtc
     2281 tcggggactt gacatatccc acatctccca gcgattggag agtctgagtg cagccaccac
     2341 ctttgagcct cttgagcctg tgaaggacac tgacattcag ggcttcctga agaatgagaa
     2401 ggacaatgcc ctgctgtctg ccatcgaaga gtcccggaag aggaccttcg gcatggctga
     2461 ggagtaccat cgggagtcaa tgttggttga gtgggagcaa gtgaaacagc gaattctgca
     2521 cacactgctg gcatcaggag aagacgccct tgactttact caagaaagcg agccaagcta
     2581 catcagtgat gtgggacccc ctggtcgaag ctctctggat aacatcgaga tggcctatgc
     2641 gcggcaaatt tatatctata atgagaaaat tgtaaatgga cacctgcagc ctaacctggt
     2701 ggacctttgt gcttccgtcg cagagctcga tgataagagc atttccgaca tgtggaccat
     2761 ggtaaaacaa atgacagacg tgttgttgac accggcaacg gatgccctga agaaccgcag
     2821 cagcgtggaa gtgcgcatgg agtttgtcag gcaggccttg gcgtaccttg agcagagtta
     2881 taagaattac acccttgtga ctgtctttgg aaatttgcat caggcccagc tgggcggggt
     2941 gcctgggact taccaattgg ttcgaagttt cctgaacatt aaactgccag ctcccttgcc
     3001 tggactacag gatggagagg tggaaggcca tcctgtgtgg gcgctaattt actactgcat
     3061 gcgctgtgga gacctgcttg ccgcttcaca ggtagttaat cgagcccagc accagctggg
     3121 agagtttaaa acctggttcc aggagtacat gaacagcaag gacagaagat tgtccccagc
     3181 tacggaaaac aagctccggc tgcattaccg tagggccctc aggaacaata cagatcccta
     3241 caagcgggcc gtgtactgta tcattggcag atgtgacgtc accgacaacc agagtgaagt
     3301 ggcggacaaa actgaggatt acctgtggct gaagttgaac caagtgtgtt ttgacgacga
     3361 tggcaccagc tccccacaag acaggctcac tctctcacag ttccagaagc agttgttgga
     3421 agactatggc gagtcccact ttacggtgaa ccagcaaccc ttcctctact tccaagtcct
     3481 gttcctgaca gcgcagtttg aagcagcagt tgcctttctt ttccgcatgg agcggctgcg
     3541 ctgccatgct gtccatgtag cactggtgct gtttgagctg aagctgcttt taaagtcctc
     3601 tggacagagg gctcagctcc tcagccacga gcctggtgac cctccttgct tgcggcggct
     3661 gaacttcgtg cggctcctca tgctgtacac ccggaagttt gagtccacgg acccaaggga
     3721 ggccctccag tacttctatt tcctcaggga tgagaaagat agtcaaggag aaaacatgtt
     3781 tctgcgctgt gtgagtgagc ttgtgattga aagccgagag ttcgatatga ttcttgggaa
     3841 actagagaat gacggaagta gaaagcctgg agtcatagat aagtttacta gtgacacaaa
     3901 gcctattatc aacaaagttg cttctgtggc agaaaataaa ggactgtttg aagaggcagc
     3961 aaagctgtat gaccttgcca agaatgctga caaggtactg gagctgatga acaaactgct
     4021 gagccctgtc gtcccccaga tcagtgcccc gcaatccaac aaggagaggc tgaagaacat
     4081 ggcactctcc attgccgaac ggtatagggc tcaaggaata agcgcaaata aatttgtgga
     4141 ctccacgttc tatcttcttt tggacttgat cacctttttt gacgagtatc atagtggtca
     4201 tattgataga gcttttgata tcattgagcg cttgaagctg gtgcccctga atcaggaaag
     4261 tgtggaagag agagtggctg ccttcagaaa tttcagtgat gaaatcaggc acaacctctc
     4321 agaagtgctt cttgccacca tgaacatctt gttcacacag tttaagaggc tcaaggggac
     4381 aagtccatcc tcgtcatcca ggccccagcg agtcatcgag gaccgcgact ctcaactccg
     4441 aagtcaagcc cgcactctga ttacctttgc tggaatgata ccataccgaa cgtctgggga
     4501 caccaatgcg aggctggtgc agatggaggt cctcatgaat gaggagctgt tcaccggggt
     4561 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4621 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4681 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     4741 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4801 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4861 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4921 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4981 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5041 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5101 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5161 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5221 cggcatggac gagctgtaca agtaagtgcc atgctttgtg ggagtctggg tcggcacact
     5281 gtcagtacat caggcacatg ggcccactag gctggggttt ctggttttgt ttctgttgtg
     5341 ttttgttttg gtttctgtat tatgtatttt tgtcaacgcc aataaatttc tttgatttgg
     5401 tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc ctgggcaata
     5461 agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag gccaagaatg
     5521 gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg cagctggccg
     5581 accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact
     5641 acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct
     5701 acttcggctt cgtgacctct agagtatccc tcgaggatcc gagctccgcg gagatccacc
     5761 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5821 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6001 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6061 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6481 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     6601 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6661 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6721 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6781 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6841 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6901 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     6961 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     7021 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7081 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7141 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     7201 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7261 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7321 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7381 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7441 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7501 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7561 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7621 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7681 gcttg
//
