LOCUS       sequence-316891-        3557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term GST 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 8 pCOLI_A_GST
FEATURES             Location/Qualifiers
     source          1..3557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             190..212
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             220..873
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     831..853
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             880..903
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1096..1114)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1110..1157
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1222..1313
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2178..2217
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      2248..2836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2737..2756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2990..3007
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3022..3161
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3087..3106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3247..3269
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3263..3454)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
       61 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttggc accatgcatc
      121 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctccggctcc
      181 ggaaataatt ttgtttaact ttaagaagga gatatacata tgtcccctat actaggttat
      241 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
      301 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
      361 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
      421 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
      481 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
      541 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
      601 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
      661 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
      721 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
      781 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
      841 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggaagttct gttccagggg
      901 cccggatccc ggtccgacat atgagcgcgc ggaattcaaa ggcctacgtc gacgagctca
      961 ctagtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt cgagaagtac
     1021 tagaggatgt acaacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg
     1081 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1141 tcttgagggg ttttttgctg aaaggaggaa ctatatcggg attagcccgc ctaatgagcg
     1201 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1381 ctcacccaga aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg
     1441 gatacataga actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc
     1501 gttttccaat gatgagcact ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag
     1561 atgcggggca agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat
     1621 actccccagt gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg
     1681 ccgccataac gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac
     1741 cgaaggaatt aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt
     1801 gggaaccaga actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg
     1861 caatggcaac aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc
     1921 aacaattaat agactggctt gaagcggata aagttgcagg accactactg cgttcggcac
     1981 ttcctgctgg ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta
     2041 tcattgccgc acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg
     2101 gtagtcaggc aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga
     2161 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg
     2221 accggtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2281 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2341 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2401 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2461 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2521 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2581 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2641 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2701 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2761 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2821 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2881 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2941 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3001 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3061 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3121 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     3181 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3241 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     3301 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     3361 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     3421 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     3481 tgttcatggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     3541 tgatgaacat gcccggt
//
