LOCUS       sequence-317035-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mature human Transforming Growth Factor alpha fused to SPM
            in bacterial cells for calcium-induced covalent coupling to 
            Epidermal Growth Factor Receptor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M
  TITLE     NeissLock provides an inducible protein anhydride for covalent 
            targeting of endogenous proteins.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):717. doi: 10.1038/s41467-021-20963-5.
  PUBMED    33514717
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):717. doi"; pages: " 
            10.1038/s41467-021-20963-5"
COMMENT     Sequence Label: pET28a-TGF-alpha-GSY-SPM
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             904..921
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtcgtaa gccattttaa tgactgtcct gactcacaca cccagttctg ttttcacggc
       61 acttgtcgct tcttagtgca agaagacaag ccagcgtgtg tctgccatag tgggtatgtt
      121 ggggctcgtt gcgaacatgc tgaccttctt gccggctcgt atgatccact ggcattagat
      181 cttgatggcg acggtatcga aacggtggca acgaaggggt ttgccggctc cttattcgat
      241 cacacaaata acggaattcg cacagctact ggatgggtaa gtgccgacga cggcctgttg
      301 gtgcgcgacc tgaatggcaa cgggattatc gacaatggcg cggaactttt tggagataac
      361 acgaaattgg cagacgggag ttttgctaag cacgggtatg cggcgttggc ggagttggat
      421 agtaacggag acaatatcat caatgcggca gatgcggcat tccagacttt gcgcgtgtgg
      481 caagatctga atcaggacgg tatttcccaa gctaatgagc ttcgtacatt ggaggagctt
      541 ggtatccagt cgttagacct ggcatacaaa gatgtcaaca agaatttagg taacggtaat
      601 actttagctc agcaaggtag ctacaccaaa accgacggaa ccactgcaaa aatgggcgac
      661 ttgttgctgg cagcagataa tctgcactcg cgtttcaagg acaaagtaga acttactgcg
      721 gaacaggcaa aggccgcaaa tctggccggt attggtcgtt tacgcgactt acgcgaggcc
      781 gcagctcttt ctggagatct ggctaatatg ttaaaagcct attcagccgc ggagaccaaa
      841 gaagcccaat tggctctgtt ggataatttg attcacaaat gggccgagac ggacggcagc
      901 agccatcatc accatcacca cagcagcggc gagccagagg cttaa
//
