LOCUS       sequence-317208-        5099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 3C cleavable CBP tag for insect cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 56 pLIB_CBP
FEATURES             Location/Qualifiers
     source          1..5099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(344..377)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        425..516
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     441..458
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             562..639
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             652..675
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    904..1038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(941..960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     995..1014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1148..1313
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1497..1951
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1584..1603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1793..1814
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1977..2081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2082..2150,2151..2942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2300..2319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3113..3701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3602..3621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3855..3872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4006..4230
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4297..4830)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSIGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5019..5024,5025..5041,5042..5047))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
       61 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
      121 gatctgccct tgcttcagga gatcggtaga cctcggccgt cgcggcgctt gccggtggtg
      181 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
      241 gcccaggact ctagctatag ttctagtggt tggctacagc tttgtttgta ctatcaacag
      301 gttgaactgc tgatcaacag atcctctacg cggccgcggt accataactt cgtatagcat
      361 acattatacg aagttatctg gttttactag tatcgattcg cgacctactc cggaatatta
      421 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
      481 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
      541 accatcgggc gcggatctat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
      601 gccaaccgct ttaagaaaat ctcatcctcc ggggcactta gcagcggatc tctggaagtt
      661 ctgttccagg ggcccggatc ccggtccgac atatgagcgc gcggaattca aaggcctacg
      721 tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct cgacaagctt
      781 gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
      841 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      901 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      961 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1021 tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc
     1081 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat aacccctagg
     1141 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     1201 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     1261 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     1321 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     1381 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     1441 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
     1501 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     1561 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     1621 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     1681 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     1741 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa tagtggactc
     1801 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1861 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1921 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2281 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2461 tgagtgataa cactgcggcc aacttacttc tgacaaccat cggtggaccg aaggaattaa
     2521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2941 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3001 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3241 cgcagatacc aaatactatt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3781 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3841 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     3901 ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt aacctggcaa
     3961 aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata
     4021 aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga
     4081 atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta
     4141 tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc
     4201 gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc
     4261 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     4321 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     4381 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     4441 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     4501 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     4561 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     4621 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     4681 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     4741 acatgtgcga atgatgccaa tacttgagcc acctaacttt gttttagggc gactgccctg
     4801 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     4861 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     4921 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     4981 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     5041 atgtcaactg ggtttgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttg
//
