LOCUS       sequence-317234-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human Rap2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-Rap2A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             571..636
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgcgcgagt acaaagtggt ggtgctgggc tcgggcgggg taggcaaatc cgccctgacc
       61 gtgcagttcg tgaccggcac cttcatcgag aaatacgacc ccaccatcga ggacttctac
      121 cgcaaggaga tcgaggtgga ttcgtcgccg tcggtgctgg agatcctgga cacggcgggc
      181 accgagcagt tcgcgtccat gcgggacctg tacatcaaga acggccaggg cttcatcctc
      241 gtctacagcc tcgtcaacca gcagagcttc caggacatca agcccatgcg ggaccagatc
      301 atccgcgtga agcggtatga gaaagtgcca gtcatcttgg ttgggaacaa agtggacctg
      361 gaaagtgaga gagaagtatc gtccagcgaa ggcagagccc ttgctgaaga gtggggctgc
      421 ccctttatgg aaacttccgc taagagtaaa acaatggtgg acgaactctt tgcagaaatt
      481 gtgaggcaga tgaactatgc tgctcagcct gacaaagatg acccatgctg ttctgcatgt
      541 aacatacaag gaggcagcgc ggccgcaggc gactacaaag accatgacgg tgattataaa
      601 gatcatgaca ttgattacaa ggatgacgat gacaagtaat aa
//
