LOCUS       sequence-317241-        2844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human HK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2457..2465
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2773..2838
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgattgcct cgcatctgct tgcctacttc ttcacggagc tcaaccatga ccaagtgcag
       61 aaggttgacc agtatctcta ccacatgcgc ctctctgatg agaccctctt ggagatctcc
      121 aaacgctttc ggaaagaaat ggagaaaggg cttggagcca ccactcaccc tactgcagca
      181 gtgaagatgc tgcccacctt tgtgaggtcc actccagatg ggacagaaca cggagagttc
      241 ctggctctgg atcttggagg gaccaacttc cgtgtgcttt gggtgaaagt aacggacaat
      301 gggctccaga aggtggagat ggagaatcag atctatgcca tccctgagga catcatgcga
      361 ggcagtggca cccagctgtt tgaccacatt gccgaatgcc tggctaactt catggataag
      421 ctacaaatca aagacaagaa gctcccactg ggttttacct tctcgttccc ctgccaccag
      481 actaaactag acgagagttt cctggtctca tggaccaagg gattcaagtc cagtggagtg
      541 gaaggcagag acgttgtggc tctgatccgg aaggccatcc agaggagagg ggactttgat
      601 atcgacattg tggctgtggt gaatgacaca gttgggacca tgatgacctg tggttatgat
      661 gaccacaact gtgagattgg tctcattgtg ggcacgggca gcaacgcctg ctacatggaa
      721 gagatgcgcc acatcgacat ggtggaaggc gatgaggggc ggatgtgcat caatatggag
      781 tggggggcct tcggggacga tggctcgctc aacgacattc gcactgagtt tgaccaggag
      841 attgacatgg gctcactgaa cccgggaaag caactgtttg agaagatgat cagtgggatg
      901 tacatggggg agctggtgag gcttatcctg gtgaagatgg ccaaggagga gctgctcttt
      961 ggggggaagc tcagcccaga gcttctcaac accggtcgct ttgagaccaa agacatctca
     1021 gacattgaag gggagaagga tggcatccgg aaggcccgtg aggtcctgat gcggttgggc
     1081 ctggacccga ctcaggagga ctgcgtggcc actcaccgga tctgccagat cgtgtccaca
     1141 cgctccgcca gcctgtgcgc agccaccctg gccgccgtgc tgcagcgcat caaggagaac
     1201 aaaggcgagg agcggctgcg ctctactatt ggggtcgacg gttccgtcta caagaaacac
     1261 ccccattttg ccaagcgtct acataagacc gtgcggcggc tggtgcccgg ctgcgatgtc
     1321 cgcttcctcc gctccgagga tggcagtggc aaaggtgcag ccatggtgac agcagtggct
     1381 taccggctgg ccgatcaaca ccgtgcccgc cagaagacat tagagcatct gcagctgagc
     1441 catgaccagc tgctggaggt caagaggagg atgaaggtag aaatggagcg aggtctgagc
     1501 aaggagactc atgccagtgc ccccgtcaag atgctgccca cctacgtgtg tgctaccccg
     1561 gacggcacag agaaagggga cttcttggcc ttggaccttg gaggaacaaa tttccgggtc
     1621 ctgctggtcc gtgttcggaa tgggaagtgg ggtggagtgg agatgcacaa caagatctac
     1681 gccatcccgc aggaggtcat gcacggcacc ggggacgagc tctttgacca cattgtccag
     1741 tgcatcgcgg acttcctcga gtacatgggc atgaagggcg tgtccctgcc tctgggtttt
     1801 accttctcct tcccctgcca gcagaacagc ctggacgaga gcatcctcct caagtggaca
     1861 aaaggcttca aggcatctgg ctgcgagggc gaggacgtgg tgaccctgct gaaggaagcg
     1921 atccaccggc gagaggagtt tgacctggat gtggttgctg tggtgaacga cacagtcgga
     1981 actatgatga cctgtggctt tgaagaccct cactgtgaag ttggcctcat tgttggcacg
     2041 ggcagcaatg cctgctacat ggaggagatg cgcaatgtgg aactggtgga aggagaagag
     2101 gggcggatgt gtgtgaacat ggaatggggg gccttcgggg acaatggatg cctagatgac
     2161 ttccgcacag aatttgatgt ggctgtggat gagctttcac tcaacccggg caagcagagg
     2221 ttcgagaaaa tgatcagtgg aatgtacctg ggtgagattg tccgtaacat tctcatcgat
     2281 ttcaccaagc gtggactact cttccgaggc cgcatctcag agcggctcaa gacaaggggc
     2341 atctttgaaa ccaagttctt gtctcagatt gagagtgact gcctggccct gctgcaagtc
     2401 cgagccatcc tgcaacactt agggcttgag agcacctgtg acgacagcat cattgttaag
     2461 gaggtgtgca ctgtggtggc ccggcgggca gcccagctct gtggcgcagg catggccgct
     2521 gtggtggaca ggatacgaga aaaccgtggg ctggacgctc tcaaagtgac agtgggtgtg
     2581 gatgggaccc tctacaagct acatcctcac tttgccaaag tcatgcatga gacagtgaag
     2641 gacctggctc cgaaatgtga tgtgtctttc ctgcagtcag aggatggcag cgggaagggg
     2701 gcggcgctca tcactgctgt ggcctgccgc atccgtgagg ctggacagcg aggaggaagt
     2761 gcggccgcag gcgactacaa agaccatgac ggtgattata aagatcatga cattgattac
     2821 aaggatgacg atgacaagta ataa
//
