LOCUS       sequence-317255-        2850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term 8xHis uncleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 3 pCOLI_A_C_HIS
FEATURES             Location/Qualifiers
     source          1..2850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..171
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        152..170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             208..230
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             290..313
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(407..425)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      421..468
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        533..624
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1489..1528
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1559..2147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2048..2067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2301..2318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2333..2472
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2398..2417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2558..2580
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2574..2765)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
       61 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
      121 gtgttggcac catgcatcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa
      181 cggtttccct ccggctccgg aaataatttt gtttaacttt aagaaggaga ctggatccga
      241 atctagagcc tgcagtctcg acaagcttgg aagcggatct ggatctggac atcatcatca
      301 tcatcaccat cactaataag tcgagaagta ctagaggatg tacaacgcgt gcgcgctgat
      361 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      421 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      481 actatatcgg gattagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
      541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctcgt gaaagtaaaa
      721 gacgcagagg accaattggg ggcacgagtg ggatacatag aactggactt gaatagcggt
      781 aaaatccttg agagttttcg ccctgaagag cgttttccaa tgatgagcac tttcaaagtt
      841 ctgctatgtg gagcagtatt atcccgtgta gatgcggggc aagagcaact cggacgacga
      901 atacactatt cgcagaatga cttggttgaa tactccccag tgacagaaaa gcaccttacg
      961 gacggaatga cggtaagaga attatgtagt gccgccataa cgatgagtga taacactgcg
     1021 gcgaacttac ttctgacaac catcggtgga ccgaaggaat taaccgcttt tttgcacaat
     1081 atgggagacc atgtaactcg ccttgaccgt tgggaaccag aactgaatga agccatacca
     1141 aacgacgagc gagacaccac aatgcctgcg gcaatggcaa caacattacg caaactatta
     1201 actggcgaac tacttactct ggcttcacgg caacaattaa tagactggct tgaagcggat
     1261 aaagttgcag gaccactact gcgttcggca cttcctgctg gctggtttat tgctgataaa
     1321 tctggggcag gagagcgtgg ttcacggggt atcattgccg cacttggacc agatggtaag
     1381 ccttcccgta tcgtagttat ctacacgacg ggtagtcagg caactatgga cgaacgaaat
     1441 agacagattg ctgaaatagg ggcttcactg attaagcatt ggtaaaccga tacaattaaa
     1501 ggctcctttt ggagcctttt tttttggacg gaccggtaga aaagatcaaa ggatcttctt
     1561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1681 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2221 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2281 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     2341 ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac
     2401 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     2461 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2521 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     2581 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     2641 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     2701 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     2761 gtcactgatg cctccgtgta agggggattt ctgttcatgg ggtaatgata ccgatgaaac
     2821 gagagaggat gctcacgata cgggttactg
//
