LOCUS       sequence-317272-       10433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mTurquoise2-tagged iLID anchored to the 
            plasma membrane via C-terminal fusion to CAAX motif from KRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Natwick DE, Collins SR
  TITLE     Optimized iLID Membrane Anchors for Local Optogenetic Protein 
            Recruitment.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 
            10.1021/acssynbio.0c00511. Epub 2021 Apr 12.
  PUBMED    33843200
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00511"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1009-1023"
COMMENT     Sequence Label: pLV_mTurquoise2-iLID-CAAX
FEATURES             Location/Qualifiers
     source          1..10433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4006..4026
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4072..4074,4075..4077,4078..4788)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4117..4138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4378..4397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4725..4746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4810..5247
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    5398..5947
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5542..5559)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5769..5788
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6402..6990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6455..6475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6873..6884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7197..7830
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7958..7974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7958..7974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7971..7993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7982..7998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8006..8012,8013..8030,8031..8036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8051..8072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8189..8206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8360..8948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8440..8459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9119..9910,9911..9979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9742..9761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9980..10084)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10132..10266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10169..10188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10223..10242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaagcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gatccgcgca aatggtgagc
     4081 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4141 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4201 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4261 accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4321 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4381 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4441 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4501 tacaactact ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     4561 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     4621 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4681 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     4741 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg tagtggtagt
     4801 ggggagtttc tggcaaccac actggaacgg atcgagaaaa atttcgtgat tactgatccg
     4861 agactgcctg acaacccaat catttttgcg agcgattcct tcctgcagct gacagaatat
     4921 tctcgggaag agatcctggg gcgcaattgc cgttttctgc agggacccga gacagaccgt
     4981 gccactgttc ggaaaatcag agatgctatt gacaaccaga ctgaagtgac cgttcagctg
     5041 atcaattata ccaagagcgg caagaagttc tggaacgtgt tccacctgca gccgatgcgc
     5101 gattataagg gcgacgtcca gtacttcatt ggcgtgcagc tggatggcac cgaacgtctt
     5161 catggcgccg ctgagcgtga ggcggtctgc ctgatcaaaa agacagcctt tcagattgct
     5221 gaggcagcga acgacgaaaa ttactttgga agcgggagtg ggagcaaaaa gaagaaaaag
     5281 aagtcaaaga caaagtgtgt aattatgtag acgcgtcggg ccgcggtaac aattgttaac
     5341 taacttaagc tagcaacggt ttccctctag cgggatcaat tccgcccccc ccccctaacg
     5401 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     5461 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     5521 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     5581 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     5641 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     5701 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     5761 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     5821 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     5881 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5941 gataatacca tggtcatgaa aacatttaac atttctcaac aagatctaga attagtagaa
     6001 gtagcgacag agaagattac aatgctttat gaggataata aacatcatgt gggagcggca
     6061 attcgtacga aaacaggaga aatcatttcg gcagtacata ttgaagcgta tataggacga
     6121 gtaactgttt gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat
     6181 tttgacacga ttgtagctgt tagacaccct tattctgacg aagtagatag aagtattcga
     6241 gtggtaagtc cttgtggtat gtgtagggag ttgatttcag actatgcacc agattgtttt
     6301 gtgttaatag aaatgaatgg caagttagtc aaaactacga ttgaagaact cattccactc
     6361 aaatataccc gaaattaacc cgggcggggc gcgtctggaa caatcaacct ctggattaca
     6421 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6481 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6541 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6601 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6661 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6721 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6781 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6841 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6901 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6961 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     7021 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7081 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7141 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     7201 agggctaatt cgctcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7261 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7321 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7381 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7441 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7501 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7561 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7621 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7801 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7861 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     7921 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8161 gtttgtgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8521 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8761 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9001 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9061 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9121 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9181 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9241 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9301 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9361 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9421 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9481 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9541 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9601 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9661 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9721 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9781 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9841 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9901 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9961 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10021 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10081 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
    10141 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10201 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10261 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10321 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10381 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
