LOCUS       sequence-317451-       14567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MUTATED_transfection of mammalian cells with Vcl mutated in 
            Tankyrase Binding Motif -II (TBM-II) under a beta-actin promoter, 
            with IRES-GFP allowing identification of transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Vilchez-Larrea, S, Valsecchi W, Fernandez-Villamil S,, Lafon Hughes 
            L
  TITLE     First evidences suggesting a role of a tankyrase-binding motif (TBM)
            of vinculin (VCL) in epithelial cells
  JOURNAL   PeerJ
REFERENCE   2  (bases 1 to 14567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ"
COMMENT     Sequence Label: Tol2-GgVCL/*TBM
FEATURES             Location/Qualifiers
     source          1..14567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6424..6448
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6450..6466
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6476..6494
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9731..9747)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9784..9802)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(9831..9855)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(9858..10432,10433..10435,10436..10444)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10025..10042)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10252..10271
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10451..10471
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10517..10519,10520..10522,10523..11236)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10517..10519,10520..10522,10523..11233)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10562..10583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10823..10842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11170..11191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(11244..11262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    11267..11401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(11464..11484)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11492..11508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    11524..11658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(12097..12284,12285..12296)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12330..12346)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(12383..12401)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12422..12438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12435..12457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12446..12462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12470..12476,12477..12494,12495..12500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12515..12536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12653..12670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12824..13412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12904..12923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13583..14374,14375..14443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14206..14225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14444..14548)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgaaa attccagttt gaagaaactt
     1141 ttcaagactc accttttcac tgttggtctt aatttaggtt gattattttt gtcaatttaa
     1201 taattacatt gtattgttca tattttattg tcttattgtg gtgtttttac agctttttta
     1261 ttgttctgct ttgttactgt ttgccttgta tgcattgtag cgctttgggt ctgagaaaag
     1321 cgcactataa ataaactgta ttattattat tagggatgga catcacggtg gcgcgatggg
     1381 tggcacaatt acctcacagc aagtaggttg ctgtttagag ccccggctgg atcagttggc
     1441 agttctgtgt ggagtttgca tgttctccct gttcaagtag gtttcctccc ggtgctctgg
     1501 tttcccctca caagtccaaa gacatgatgt ataggtgaat ggggtaagct aaattgacca
     1561 tagtgagtgt gtgtgtgtgt gtgaatgaga gtgtataggt gtttcccagt gttaggttgc
     1621 aactggaagg acatctgctg tgtaaaacat atgcaggata agttggcggt tcattcccct
     1681 atggtgaccc ctggttaatt aaaggactaa gttgaaaatg aatgaatgaa tgaatgatta
     1741 gagataaacc gataccactt ttttacaaac cgatatgagt acaagtataa tagtttctgt
     1801 tactcgacaa tactgagtac cgattctgat acttcttgga tttggtttca atgtaattaa
     1861 tttgaaagaa ggttttattt cttgcctgtc gcaaggatga acacaaaaat ctttaccaga
     1921 aggatatttt aagctaagaa tatacgcacg ttgttgacaa ccctgtatcg attaccgatt
     1981 aaacgtggac atttaaagga taagttatgc ttgcgccatc acaactttac acattacatg
     2041 atcaaagatc atattgaata ttcggtttat tcgtcagaag cctatagatt tgttaaaagt
     2101 gtattttata aacggatggt ttataaagac taagctatta atatatcaga cattcattaa
     2161 tacatcaaga catcgatgtc aggtaggaca acaaatcaca tccatgtaat gaaatcttgt
     2221 tgagtggtat tggtgcgtgg tttcagcatt tcatgtgcaa tacgagtaag aatattttaa
     2281 ccaagtatcg acccaaaacc tgatactggt atcggtgcat cccttattat tattattatc
     2341 aaaaacacac ttgttttatt tatgtgggtg ccaaacaaca aactatgaaa tgcagcatga
     2401 cacgcccaaa aagtgttcct ttgagaacaa cttcataaat atgtttctta tcattatgca
     2461 tgaatggtgt actgggagag ctttggtatc agaattgaga taagagactt tggcattcta
     2521 aaggacaatg acattatcac actgacagtc aacaacactt tattgctctg gaaatcttat
     2581 gtcatggggc aaagctgagg ttcattagga cagtacctta tttagagatg aatgggaagg
     2641 ctgtagtggg ctgctatcag ggaagggtgg tggtcctcgg gggaactatg gactcacaaa
     2701 agcacatctg ctgtaacaag atcccctgtg caatctacaa aagttacaaa gagaagcatg
     2761 gttggttaaa aaatagaatt gactggatat gctgaatgca ttgtttgttg tgtgttacat
     2821 cacattaacc acagattggt gctaaatatt tgcttatctt ataaaaacct gtgtgtttat
     2881 aaaggggggt gtggatggat gcttggcatt ctttgaaaag atgttggggg atggaaggag
     2941 gagaccttat tgatgagtat ccagtttgca gataacaagt ttttagacat tcatactatg
     3001 atgctgttta tgtctgtttt ttctcatttt ttctatacac aaacagctat aaaacattca
     3061 cttttatatc tgatgctgta tattaaattt cgttgtattt attctgtttg ctctgttggg
     3121 tcatagctag ggccagacag aatctgctga cattttttgc tttttctgca gagaattttg
     3181 taaacaatct gtgatttatg cgagattttt tcgggagtat cgtaactaaa aacttactat
     3241 atgaaataaa aaaaaataat acctttttaa cttttaataa atatatacaa tgcaagtcca
     3301 cctagatcca attatttggt cgacaaagca agtctctcac ataatatatc ttctaaaaga
     3361 cagaaaacat tactttcaga ccatattgta atcaaatgaa cactttcata ttagtcaata
     3421 atattacgga aatgtattta aaaactgaat aaacatatat ttacaccttt gtctttattt
     3481 ctagagtgct tttacaatgt agattgtgtc aaagcagctt aacttagagg attagtatag
     3541 taaattgaaa ctgtgtcagt ccatttttta agagtttaag ttcagttcag tttagttcag
     3601 tgtgagttaa tttttactgc tgagagtcca aacactaaag agcaaatcca aatacacaag
     3661 tcaataaata aactcaatga tgggctaaag atctggggaa atctgcagat ttctgtgcgc
     3721 acagattctg tgtgggccta gtcattacct atactcaacg tttactctga gaaacttcca
     3781 tattttagag gtaaaatgct cagaattgta aaaagtcttg tcaaaatact tggaatttgc
     3841 caaaaacaaa ataaaagtca catcacctca ttgtccttgg tatcagcaag cacttttaac
     3901 acaatcagct gtttttgttt ataactccca ctgaaggcca tgtgcattac tcccaagtta
     3961 cacagtaaaa agtcacatgt agtttatcat gatcactgct gctcaagcac agctttatct
     4021 gttattcagt gtggcaatat ctgaaatgtg tgcaaaatat tatttattgt agctgatcac
     4081 caacactttc agtgtttttt ttttctactt ttaatgagaa ttatgttctt aggatttatc
     4141 cttataaacc tctttaaaaa gaaatgttat gtctgcatag acacgacatg aaattatgaa
     4201 cagaaaattt aactatttaa gatcaagact tgacatataa catttctaat gaacattaaa
     4261 cctgaggtag gcccacttgc aaaaatgtaa ctgctatcac tcactttata catcattgtg
     4321 taacttgtac acacaaaaat agtgaagcaa tgtggcatgg aatgcaggtc ccattcattc
     4381 aaagttatta aagcactttc ttaaagtgca ttactactga gctaagacca gctagttgct
     4441 catacaacat actacaagta gtgtcatcct aatttggata aaaaaaatgt ccattttata
     4501 catggaaaag tactcattag atcccccatt gtttgtatta cggtatttcg tgaacacaag
     4561 aggtaaatga cctacagagc tgctgttgtg ttagattgaa aacacaacac aggatcatgg
     4621 aggcatccat cattatgacg cattctgcca cttgtaactc tgcacagact ttttgaaaag
     4681 tttaattgag tcttggcgtt gtcgcagaaa aatacgaaaa attctgtgtt aaaatttgca
     4741 aataaaaatt ccagttttat aggaaatcgc catggccttg ttctttaacc agttaagctt
     4801 ccccttcttt cactctcaag ttgcaagaag caagtgtagc aatgtgcacg cgacagccgg
     4861 gtgtgtgacg ctggaccaat cagagcacag agctccgaaa gtttaccttt tatggctaga
     4921 gccgggtatg tgccgtcata taaaagagcg cgcccagctt ttcagcctca ctttgagctc
     4981 ctccacacgc agctagtgcg gaatatcatc tgcttgtaac ccattctctt aagtcgacaa
     5041 ccccccaaac ccaaggtgag ttggttttac agtttttaag cgtttaatta tagtttctat
     5101 ctattaatgt taagtaaaat gtgtaaatgg ataaatatga tttgatgaag tctttagaca
     5161 gttcataacg gtctctttgt agttatctac taggtttgat ttataagtgg tggttctttt
     5221 gataatctat gttacattta ataaatattg gctgtatctt aacttttttg tagcgttagt
     5281 ggatgtggca ggtgagaatg tcggtaattt aacgtgacca gtcgtaggca cgacattgaa
     5341 cattgaatag gccggtgtga aatgggtgtt aagtcttaat ataaagttgt gttctggcta
     5401 atctctcttt aacagccata aatgtgattt ttaactacag ttttgagggt gctggtgagc
     5461 atgttgcaca cttgatggat agccggcatg ggaagttctt tgtgcaggca gtgctgcagc
     5521 agggtgtgac ctactttagc tagccggcta accagctctc atctgatgta acgtaaaccc
     5581 catcaatcta acatcggctc attcgtgctt tataagatcg aagatcttgt tattagtaac
     5641 gggtattggc atttagtttt aacttggtta attttctttt tgttatgtgt tctatttaaa
     5701 tgtagcctgc agccccctct gtctttaatg aagctgaaga ttaacgttaa cgttagtgaa
     5761 gtgttgtatc cgctatgaaa gatttgaatt ttagaggtgt ggtccacttt aataacggct
     5821 gcggagttct ctggctgcac ttcagccgct gtgactgtcg aagcagagcc catcagtttt
     5881 tcaggatctc ggtgattttt gaggctagac tatgaactga accgactgag agtgaccgtc
     5941 cagaaatgct ttagtgtgaa gtctccgctc acgaggagtc cgtggactga ctgcagatct
     6001 gtgacgcagt aacacccggc ggcagacacc cgttgaaatg cggttgtgta attgatcgca
     6061 ggcgagggtg gaagaggatg tgaaacttca tttgtgtaaa atttagggag tggtcctggt
     6121 gtgatgaatg tcgaaatctg ttccttttta ctgagcccta cgactctggc tgagtgccac
     6181 accgccggca gccgcaaagc ggctcaaacc attgcctttt atggtaataa tgagagaatg
     6241 cagagggact tcctttgtct ggcatatctg aggcgcgcgc tgtcactagc gcccaccagc
     6301 ggtcagactg tagaatgcag agcaaaacag gaagttgact ccagatggtc acatgcttgc
     6361 tgaaacgcct ctccctggca gcagtgcaat tctaacagtt tccctttctt ttacagttca
     6421 gcctcaagtt tgtacaaaaa agcaggcttg taaaacgacg gccagtgagc gcgcgtaata
     6481 cgactcacta tagggcgaat tgggtaccat gatgcccgtc ttccacacgc gcaccatcga
     6541 gagcatcttg gagcccgtgg ctcagcagat ctcccacctg gtcatcatgc acgaggaggg
     6601 ggaggtagac ggcaaggcca tcccggacct caccgccccc gtgtcggccg tgcaggccgc
     6661 tgtcagcaac ctggtgcggg ttggaaaaga aactgtgcag acaacagaag accagatctt
     6721 gaaaagggat atgccaccag cattcatcaa agtagagaat gcctgcacca agctcgttcg
     6781 agcagcccag atgctgcaag cagatcctta ttcagtacca gctcgtgact acctaattga
     6841 tggatcaaga ggcatccttt ctggaacatc agacttactt ctgacatttg atgaagcgga
     6901 ggtccgtaaa atcatccgtg tctgcaaagg aatattggaa tatctgactg tggcagaagt
     6961 agtagagact atggaggatt tggtgacata tacaaagaat ctagggccag gaatgacaaa
     7021 gatggcgaaa atgatcgatg agagacaaca ggaattaact catcaggaac atagggttat
     7081 gctggtgaac tccatgaata ctgtgaagga gctattgcct gtacttattt cagctatgaa
     7141 gatctttgta accacaaaaa acactaaaag ccagggaata gaagaggcct tgaaaaatcg
     7201 caatttcaca gtagagaaaa tgagtgctga gataaatgaa ataatccgtg tattacaact
     7261 cacttcctgg gatgaagatg cctgggccag caaggacact gaagccatga aaagagcttt
     7321 agccctaata gattcaaaga tgaaccaggc aaaaggctgg ctgagagatc caaacgcacc
     7381 tccaggggat gctggtgagc aagcaataag gcagatcctt gatgaagctg gaaaagcagg
     7441 agaattgtgt gcaggcaaag aacgcaggga gattctggga acatgcaaaa ctttgggcca
     7501 aatgactgat caacttgctg atctccgagc tagaggacag ggtgctacac cgatggcaat
     7561 gcagaaggca cagcaggtgt cacaaggcct ggatttgctc actgcaaaag tggagaatgc
     7621 agcccggaaa ttggaggcca tgacaaactc taagcaggct attgcaaaga agattgatgc
     7681 tgctcagaac tggcttgcgg atcccaacgg cggaagtgaa ggagaagaac acattcgagg
     7741 aattatgtct gaagcaagga aagttgcaga attgtgtgag gagcctaaag aaagagatga
     7801 tatccttcgc tccttggggg aaatctctgc tctgacagct aagctgtcag atctgcgacg
     7861 acatgggaaa gtagactctc ctgaggcccg tgcattggcc aagcaaatag ctacatcact
     7921 tcagaactta cagtccaaaa caaacagagc tgtagcaaat actaggccag ttaaagctgc
     7981 tgtccatttg gagggcaaga ttgagcaagc ccagaggtgg atagataatc ctacagttga
     8041 tgatcgggga gtaggccagg cagcaattcg gggtttggtt gcagaaggtc gtcgtctagc
     8101 caatgtcatg atgggacctt atcgtcagga cctgcttgcc aaatgtgacc gtgtagacca
     8161 gctggctgct cagcttgctg accttgcagc aagaggggaa ggagagtctc ctcaggctag
     8221 ggcaattgct gctcagcttc aggactccct gaaggatctc aaagcacgga tgcaagaagc
     8281 aatgacccag gaggtgtctg atgttttcag tgacactaca acccctatta agttgttagc
     8341 agtagcagcc actgctcctt ctgatactcc caatagagaa gaggtgtttg aagaaagagc
     8401 agcaaatttt gaaaaccatg ctgctagact gggagcaaca gcagaaaaag cagctgcagt
     8461 tggaactgct aataaaacta ctgtggaagg cattcaggca acagtcaaat ctgcaaggga
     8521 gcttacacca caggtagtat cggctgctcg aatcctcctg agaaatcctg gaaatcaagc
     8581 tgcttatgag cattttgaga caatgaaaaa ccaatggatt gataatgtag aaaagatgac
     8641 agggctggtg gatgaggcca tcgataccaa gtctctgttg gatgcatcag aagaggctat
     8701 taagaaggat cttgataaat gcaaagttgc aatggccaat atgcaacctc agatgctggt
     8761 agctggagcc accagcattg ctagacgagc gaaccgcatc ctgcttgtgg caaaacggga
     8821 ggttgaaaat tcagaagacc ctaaattcag ggaggctgtt aaagcagctt ctgatgagct
     8881 gagcaaaacc atatcaccga tggtaatgga tgctaaagct gtagcaggaa atatctctga
     8941 tcctggtttg cagaagagtt tcttggattc tggatacagg attctgggag ctgtggccaa
     9001 agtcagagaa gcctttcagc ctcaggagcc agactttccc cctcctcctc ctgacctaga
     9061 gcatctccat ctgactgatg agcttgctcc tccaaaacca ccacttccag aaggtgaggt
     9121 tcccccaccc agaccaccac cccctgaaga aaaagatgag gagttcccag agcagaaagc
     9181 aggagaagct attaatcagc ccatgatgat ggctgctagg cagttgcatg acgaggctcg
     9241 gaaatggtct agcaagggta acgacatcat tgctgctgct aaacgaatgg cgctgctcat
     9301 ggctgagatg tcacgcctgg tgcgaggagg cagcggaaac aagcgtgccc tcatccagtg
     9361 tgcaaaagat attgctaagg catcggatga agtcactcga ttggccaaag aggtggccaa
     9421 gcaatgtact gataaacgca ttagaacaaa cctcttacag gtctgtgagc gaatcccaac
     9481 catcagcacg cagctcaaaa ttctttccac agtgaaagct accatgctgg gcaggactaa
     9541 catcagcgat gaagaatcag aacaggcaac tgagatgttg gttcataacg cccagaacct
     9601 catgcagtct gtgaaggaaa ctgtgagaga agctgaagca gcatccatta agataagaac
     9661 agatgccgga ttcactctgc gctgggtcag aaagacccca tggtatcagt agaattcctg
     9721 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     9781 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc acccagcttt
     9841 cttgtacaaa gtggccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     9901 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     9961 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
    10021 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
    10081 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
    10141 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
    10201 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
    10261 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
    10321 gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
    10381 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
    10441 aaccatggct ccaaagaaga agcgtaaggt aaatcgatcg aattcaaggc ctctcgagcc
    10501 tctagaaggt ggcggaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
    10561 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
    10621 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    10681 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
    10741 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
    10801 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
    10861 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
    10921 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
    10981 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
    11041 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
    11101 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
    11161 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
    11221 cgagctgtac aagtaactgc agccctatag tgagtcgtat tacgtagatc cagacatgat
    11281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
    11341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
    11401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
    11461 ttccaacttt attatacata gttgataatt cactggccgt cgttttacgg taccatcgat
    11521 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    11581 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    11641 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
    11701 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
    11761 gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
    11821 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
    11881 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
    11941 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
    12001 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
    12061 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
    12121 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
    12181 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
    12241 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
    12301 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
    12361 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    12421 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    12481 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    12541 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    12601 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    12661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    12721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    12781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    12841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    12901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    12961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    13021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    13081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    13141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    13201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    13261 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    13321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    13381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    13441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    13501 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    13561 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    13621 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    13681 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    13741 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    13801 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    13861 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    13921 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    13981 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    14041 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    14101 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    14161 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    14221 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    14281 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    14341 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    14401 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    14461 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    14521 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
