LOCUS       sequence-31749-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Bommer GT, Feng Y, Iura A, Giordano TJ, Kuick R, Kadikoy H, Sikorski
            D, Wu R, Cho KR, Fearon ER
  TITLE     IRS1 regulation by Wnt/beta-catenin signaling and varied 
            contribution of IRS1 to the neoplastic phenotype.
  JOURNAL   J Biol Chem. 2010 Jan 15. 285(3):1928-38.
  PUBMED    19843521
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jan 15. 285(3):1928-38."
COMMENT     Sequence Label: IRS1-B1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     41..57
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(189..208)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        354..853
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(389..412)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     786..805
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atcgatctct cgaggtcgac ggtatcgata
       61 agcttaaaaa gctatgctga catgcgaaca tgacaggaag tattcacatg tcagcatagc
      121 gggatctcta tcactgatag ggaacttata agattcccaa atccaaagac atttcacgtt
      181 tatggtgatt tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt
      241 ccctcacagc catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac
      301 tgggcccgcg attccttgga gcgggttgat gacgtcagcg ttcgaattct accgggtagg
      361 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
      421 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
      481 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
      541 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
      601 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
      661 cggccaatag cagctttgct ccttcgcttt ctgggctcag angctgggaa ggggtgggtc
      721 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
      781 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
      841 tccgggcctt tcgacctgca gcccaagcta gcttaccatg ac
//
