LOCUS       sequence-317542-        8717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stable integration of FLAG-METTL3 in human cells with 
            trasposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Sorci M, Ianniello Z, Cruciani S, Larivera S, Ginistrelli LC, 
            Capuano E, Marchioni M, Fazi F, Fatica A
  TITLE     METTL3 regulates WTAP protein homeostasis.
  JOURNAL   Cell Death Dis. 2018 Jul 23;9(8):796. doi: 
            10.1038/s41419-018-0843-z.
  PUBMED    30038300
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2018-07-23"; volume: "9(8):796. doi"; pages: " 
            10.1038/s41419-018-0843-z"
COMMENT     Sequence Label: ePB-PURO-FLAG-METTL3
FEATURES             Location/Qualifiers
     source          1..8717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             69..92
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1954..2075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2261..2323
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2358..2377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2359..2377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2387..2404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2387..2403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2396..2418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2545..3000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2632..2651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2842..2863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3026..3130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3131..3199,3200..3991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3349..3368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4162..4750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4651..4670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4904..4921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5038..5059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5074..5079,5080..5097,5098..5104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5112..5128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5117..5139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5136..5152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5136..5152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5171..5191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5173..5191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5239..5273
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5561..5682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5729..6328)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5812..5832)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(6332..6385)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(6386..6391,6392..6427,6428..6430,6431..6466,
                     6467..6469,6470..6505,6506..6511,6512..7129))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7148..7195)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7204..8415)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7348..7367)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        join(8431..8717,1..28)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8439..8457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8475..8493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8510..8528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8546..8564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8582..8600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8617..8635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8653..8671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagaattcga gctcggtacc cggggatcca
       61 agcttatgga ttacaaggat gacgacgata agtcggacac gtggagctct atccaggccc
      121 acaagaagca gctggactct ctgcgggaga ggctgcagcg gaggcggaag caggactcgg
      181 ggcacttgga tctacggaat ccagaggcag cattgtctcc aaccttccgt agtgacagcc
      241 cagtgcctac tgcacccacc tctggtggcc ctaagcccag cacagcttca gcagttcctg
      301 aattagctac agatcctgag ttagagaaga agttgctaca ccacctctct gatctggcct
      361 taacattgcc cactgatgct gtgtccatct gtcttgccat ctccacgcca gatgctcctg
      421 ccactcaaga tggggtagaa agcctcctgc agaagtttgc agctcaggag ttgattgagg
      481 taaagcgagg tctcctacaa gatgatgcac atcctactct tgtaacctat gctgaccatt
      541 ccaagctctc tgccatgatg ggtgctgtgg cagaaaagaa gggccctggg gaggtagcag
      601 ggactgtcac agggcagaag cggcgtgcag aacaggactc gactacagta gctgcctttg
      661 ccagttcgtt agtctctggt ctgaactctt cagcatcgga accagcaaag gagccagcca
      721 agaaatcaag gaaacatgct gcctcagatg ttgatctgga gatagagagc cttctgaacc
      781 aacagtccac taaggaacaa cagagcaaga aggtcagtca ggagatccta gagctattaa
      841 atactacaac agccaaggaa caatccattg ttgaaaaatt tcgctctcga ggtcgggccc
      901 aagtgcaaga attctgtgac tatggaacca aggaggagtg catgaaagcc agtgatgctg
      961 atcgaccctg tcgcaagctg cacttcagac gaattatcaa taaacacact gatgagtctt
     1021 taggtgactg ctctttcctt aatacatgtt tccacatgga tacctgcaag tatgttcact
     1081 atgaaattga tgcttgcatg gattctgagg cccctggcag caaagaccac acgccaagcc
     1141 aggagcttgc tcttacacag agtgtcggag gtgattccag tgcagaccga ctcttcccac
     1201 ctcagtggat ctgttgtgat atccgctacc tggacgtcag tatcttgggc aagtttgcag
     1261 ttgtgatggc tgacccaccc tgggatattc acatggaact gccctatggg accctgacag
     1321 atgatgagat gcgcaggctc aacatacccg tactacagga tgatggcttt ctcttcctct
     1381 gggtcacagg cagggccatg gagttgggga gagaatgtct aaacctctgg gggtatgaac
     1441 gggtagatga aattatttgg gtgaagacaa atcaactgca acgcatcatt cggacaggcc
     1501 gtacaggtca ctggttgaac catgggaagg aacactgctt ggttggtgtc aaaggaaatc
     1561 cccaaggctt caaccagggt ctggattgtg atgtgatcgt agctgaggtt cgttccacca
     1621 gtcataaacc agatgaaatc tatggcatga ttgaaagact atctcctggc actcgcaaga
     1681 ttgagttatt tggacgacca cacaatgtgc aacccaactg gatcaccctt ggaaaccaac
     1741 tggatgggat ccacctacta gacccagatg tggttgcacg gttcaagcaa aggtacccag
     1801 atggtatcat ctctaaacct aagaatttat aggcggccgc gactctagat cataatcagc
     1861 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1921 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2041 agttgtggtt tgtccaaact catcaatgta tcttaaggaa ttcgataaaa gttttgttac
     2101 tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat
     2161 tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc
     2221 aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat
     2281 gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct agctgcggcg
     2341 cgccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2401 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2461 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2521 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2581 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2641 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2701 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2761 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2821 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2881 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2941 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggggc
     5221 gcgccgcagc tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt
     5281 gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc
     5341 agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt
     5401 gaactataac aaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag
     5461 atttaactca tacgataatt atattgttat ttcgtgttct acttacgtga taacttatta
     5521 tatatatatt ttcttgttat agatgggaat tcagacatga taagatacat tgatgagttt
     5581 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     5641 attgctttat ttgtaaccat tataagctgc aataaacaag tttcgaggtc gagtgtcagt
     5701 cctgctcctc ggccacgaag tgcttagctc aggcaccggg cttgcgggtc atgcaccagg
     5761 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     5821 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
     5881 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     5941 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     6001 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     6061 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     6121 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     6181 gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc gtggcggggt
     6241 agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg cgggtggcga
     6301 ggcgcaccgt gggcttgtac tcggccatgg tagggccggg attctcctcc acgtcaccgc
     6361 atgttagaag acttcctctg ccctccccgg ggagcatgtc aaggtcaaaa tcgtcaagag
     6421 cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc atcggctggg agcatgtcta
     6481 agtcaaaatc gtcaagggcg tcggccggcc cgccgctttc gcactttagc tgtttctcca
     6541 ggccacatat gattagttcc aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga
     6601 acagctcaat tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt gtctctcttt
     6661 cctcttttgc tacttgatgc tcctgttcct ccaatacgca gcccagtgta aagtggccca
     6721 cggcggacag agcgtacagt gcgttctcca gggagaagcc ttgctgacac aggaacgcga
     6781 gctgattttc cagggtttcg tactgtttct ctgttgggcg ggtgccgaga tgcactttag
     6841 ccccgtcgcg atgtgagagg agagcacagc ggaatgactt ggcgttgttc cgcagaaagt
     6901 cttgccatga ctcgccttcc agggggcaga agtgggtatg atgcctgtcc agcatctcga
     6961 ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct
     7021 caactcccag cttttgagcg agtttccttg tcgtcaggcc ttcgataccg actccattga
     7081 gtaattccag agcgccgttt atgactttgc tcttgtccag tctagacatg gtttagttcc
     7141 tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg tcaacacggt
     7201 ccggtctaac aaaaaagcca aaaacggcca gaatttagcg gacaatttac tagtctaaca
     7261 ctgaaaatta catattgacc caaatgatta catttcaaaa ggtgcctaaa aaacttcaca
     7321 aaacacactc gccaaccccg agcgcatagt tcaaaaccgg agcttcagct acttaagaag
     7381 ataggtacat aaaaccgacc aaagaaactg acgcctcact tatccctccc ctcaccagag
     7441 gtccggcgcc tgtcgattca ggagagccta ccctaggccc gaaccctgcg tcctgcgacg
     7501 gagaaaagcc taccgcacac ctaccggcag gtggccccac cctgcattat aagccaacag
     7561 aacgggtgac gtcacgacac gacgagggcg cgcgctccca aaggtacggg tgcactgccc
     7621 aacggcaccg ccataactgc cgcccccgca acagacgaca aaccgagttc tccagtcagt
     7681 gacaaacttc acgtcagggt ccccagatgg tgccccagcc catctcaccc gaataagagc
     7741 tttcccgcat tagcgaaggc ctcaagacct tgggttcttg ccgcccacca tgccccccac
     7801 cttgtttcaa cgacctcaca gcccgcctca caagcgtctt ccattcaaga ctcgggaaca
     7861 gccgccattt tgctgcgctc cccccaaccc ccagttcagg gcaaccttgc tcgcggaccc
     7921 agactacagc ccttggcggt ctctccacac gcttccgtcc caccgagcgg cccggcggcc
     7981 acgaaagccc cggccagccc agcagcccgc tactcaccaa gtgacgatca cagcgatcca
     8041 caaacaagaa ccgcgaccca aatcccggct gcgacggaac tagctgtgcc acacccggcg
     8101 cgtccttata taatcatcgg cgttcaccgc cccacggaga tccctccgca gaatcgccga
     8161 gaagggacta cttttcctcg cctgttccgc tctctggaaa gaaaaccagt gccctagagt
     8221 cacccaagtc ccgtcctaaa atgtccttct gctgatactg gggttctaag gccgagtctt
     8281 atgagcagcg ggccgctgtc ctgagcgtcc gggcggaagg atcaggacgc tcgctgcgcc
     8341 cttcgtctga cgtggcagcg ctcgccgtga ggaggggggc gcccgcgggg ggcgccaaaa
     8401 cccggcgcgg aggccagatc tcccgatctc gagtttactc cctatcagtg atagagaacg
     8461 tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt
     8521 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     8581 ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta
     8641 tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     8701 gaggcctata taagcag
//
