LOCUS       sequence-31784-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector with human U6 promoter driving mouse G alpha 12 
            miR30-based shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_hU6miR-G12-E
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           87..208
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           236..250
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           278..404
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(437..536)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(648..667)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(726..745)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctttatatat cttgtggaaa ggacgaaaca ccgtgctcgc ttcggcagca catatactag
       61 tcgactaggg ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg
      121 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
      181 cgagaaggta tattgctgtt gacagtgagc gacgacacca tcttcgacaa cattagtgaa
      241 gccacagatg taatgttgtc gaagatggtg tcgctgccta ctgcctcgca attcaagggg
      301 ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac
      361 atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa ttcgcggccg
      421 cactcgagat atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
      481 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      541 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      601 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      661 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
      721 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
      781 gtatgggaag cccgatgcgc caga
//
