LOCUS       sequence-31792-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector withTRE promoter driving control Luciferase miR30-based
            shRNA with GFP coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pEN_TGmiR_Luc-A
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           47..168
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           196..210
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..364
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(403..502)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(614..633)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(692..711)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gagctgtaca agtaaactag tcgactaggg ataacagggt aattgtttga atgaggcttc
       61 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
      121 ggttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gcccgcctga
      181 agtctctgat taatagtgaa gccacagatg tattaatcag agacttcagg cggttgccta
      241 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
      301 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
      361 cttttttcaa ttggaagact aatgcgtttt cgagatatct agacccagct ttcttgtaca
      421 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      481 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
      541 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      601 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
      661 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      721 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
      781 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatg
//
