LOCUS       sequence-31797-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, 
            Lieberman J
  TITLE     miR-34a contributes to megakaryocytic differentiation of K562 cells 
            independently of p53.
  JOURNAL   Blood. 2009 Sep 3. 114(10):2181-92.
  PUBMED    19584398
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2009
            Sep 3. 114(10):2181-92."
COMMENT     Sequence Label: pSIREN-RetroQ-CDK6-shRNA
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        263..762
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(298..321)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     695..714
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(783..802)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aggatccgca tgtcgatcaa gacttgattc aagagatcaa gtcttgatcg acatgttttt
      241 tgaattcttt tttaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
      301 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
      361 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
      421 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
      481 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
      541 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
      601 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
      661 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
      721 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
      781 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
      841 tacgcaccct c
//
