LOCUS       sequence-318-exp        4394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4394)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD117.20
FEATURES             Location/Qualifiers
     source          1..4394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(614..642)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1390..1410
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1968..1990)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2090..2109
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2187..2205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2273..2377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2378..2446,2447..3238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2596..2615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3409..3997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3898..3917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4151..4168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4285..4306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4321..4326,4327..4344,4345..4351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4359..4375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4364..4386
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4383..4394,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4383..4394,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctagaggatc ctcgtgttac
       61 ttgaatatca aacttattgg atacattcat cttctacact cgggctcaaa agagctcaaa
      121 acaaatgtgc tctatcgaca actcctcccc ccattcggtc acccattcag cgtcgcattc
      181 atcgccgcgc gttcgtcctc ctcgacacca atacctcctt cattcttcaa cgttagtggg
      241 gctatatgac cagtcgtttt gcaattaatg tatgattctc tccgtctaca ccactggaaa
      301 cccatgtcct ctttctttgt ttcttcgtct cgttgtcaac gtctattcaa accacgtgct
      361 atttcttgag ttttgttgtc ttctcccaat ccatcactag tctgttcttc cttgcatttt
      421 tttgatcgaa tttttaaaac tcctctcata atttttatgt taataattca ctatccactt
      481 gattgtattt aattttcagt aaaaataaag gcatcggcta gaggatcccc gggattggcc
      541 aaaggaccca aaggaggacc cttggagggt accggtagaa aaaatgagta aaggagaaga
      601 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      661 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      721 tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac
      781 taaccctgat tatttaaatt ttcagccaac acttgtcact actctcactt atggtgttca
      841 atgcttctcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      901 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      961 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt
     1021 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     1081 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     1141 catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac
     1201 taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag
     1261 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1321 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1381 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggacga
     1441 actatacaaa agcccacaag ctttggaatt cgctagccgg ccatacaagt aatccggatg
     1501 atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct
     1561 attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca
     1621 aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg
     1681 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat
     1741 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc
     1801 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga
     1861 ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg agaattgtcc
     1921 tcgcttgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1981 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     2041 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     2101 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     2161 aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt
     2221 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     2281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3001 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3541 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3781 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4081 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4141 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     4201 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4261 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     4321 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     4381 cacaggaaac agct
//
