LOCUS       sequence-31804-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De 
            Robertis EM
  TITLE     Isthmin is a novel secreted protein expressed as part of the Fgf-8 
            synexpression group in the Xenopus midbrain-hindbrain organizer.
  JOURNAL   Mech Dev. 2002 Aug . 116(1-2):169-72.
  PUBMED    12128218
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev. 
            2002 Aug . 116(1-2):169-72."
COMMENT     Sequence Label: pCS2 xenopus Insulin 2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    382..516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(406..425)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     460..479
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(629..649)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(668..684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(668..684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(681..703)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    692..708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(716..722,723..740,741..746))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    761..782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccatcgattc gaattcccac tatcttttac aatggctcta tggatgcagt gtctgcccct
       61 ggttcttgtc ctccttttct ctacacccaa caccgaagct ctagccaacc agcacttgtg
      121 tgggtctcac ctagtagaag ccctgtacct agtatgtggg gatagaggct tcttctacta
      181 ccctaagatc aaacgggaca tcgaacaagc acaggtcaat ggaccccaag acaacgagtt
      241 agatggaatg cagttccagc ctcaagagta tcagaaaatg aagaggggga ttgtggagca
      301 atgctgccac agcacatgtt ctctcttcca gctggagaac tactgcaact agtctagaac
      361 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
      421 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      481 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      541 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg
      601 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      661 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      721 tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      781 taattgcgtt gcgctcactg cccgctttcc agtcgggaa
//
