LOCUS       sequence-318148-        8859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin 
            resistant. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Rovira-Clave X, Drainas AP, Jiang S, Bai Y, Baron M, Zhu B, Dallas 
            AE, Lee MC, Chu TP, Holzem A, Ayyagari R, Bhattacharya D, McCaffrey 
            EF, Greenwald NF, Markovic M, Coles GL, Angelo M, Bassik MC, Sage J,
            Nolan GP
  TITLE     Spatial epitope barcoding reveals clonal tumor patch behaviors.
  JOURNAL   Cancer Cell. 2022 Nov 14;40(11):1423-1439.e11. doi: 
            10.1016/j.ccell.2022.09.014. Epub 2022 Oct 13.
  PUBMED    36240778
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2022.09.014"; journalName: "Cancer Cell"; date: 
            "2022-11-14- 14"; volume: "40"; issue: "11"; pages: "1423-1439.e11"
COMMENT     Sequence Label: pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG
FEATURES             Location/Qualifiers
     source          1..8859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1210..1806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1210..1229)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1706..1726
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1816..1869
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1870..1872,1873..1875,1876..2586)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1870..1872,1873..1875,1876..2586)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1915..1936)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2176..2195)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2523..2544
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2617..2649
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             2650..2676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2650..2676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2677..2700
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2704..2728)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2746..3334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2799..2819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3217..3228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3422..3655
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3727..3848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3764..3783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3818..3837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3888..4023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4043..4062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4044..4062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4072..4089)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4072..4088)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4081..4103)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4230..4685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4317..4336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4527..4548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4711..4815
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4816..4884,4885..5676)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5034..5053)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5847..6435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6336..6355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6589..6606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6723..6744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6759..6764,6765..6782,6783..6789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6797..6813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6802..6824
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6821..6837
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6821..6837
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6856..6876
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6858..6876
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6904..7130
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7131..7311
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7358..7483
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7976..8209
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8394..8438
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8704..8821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8839..8859
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac aagtttgtac aaaaaagcag
     1201 gctgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     1261 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     1321 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     1381 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     1441 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     1501 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     1561 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     1621 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     1681 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     1741 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     1801 ggtgccggaa gcggagaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat
     1861 cccggcccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1921 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1981 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2041 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2101 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2161 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2221 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2281 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2341 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2401 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2461 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2521 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2581 tacaagggtg gcagcggcgg cggcggctct ggcggctaca ccgacatcga gatgaatcgt
     2641 ctgggcaagt acccctacga cgtgcccgac tacgccgact acaaagacga tgacgacaag
     2701 taaacccagc tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat
     2761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3121 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3301 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgcttta
     3361 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3421 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     3661 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3721 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3781 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3841 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3961 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4021 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     4081 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4141 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4201 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4261 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4321 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4681 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4741 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4801 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4861 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4921 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4981 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5041 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5101 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5161 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5221 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5281 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5341 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5401 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5461 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5521 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5581 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5641 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5701 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5761 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5821 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5881 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5941 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6001 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6061 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6241 tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca ggtatccggt aagcggcagg
     6301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6541 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6601 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6661 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6721 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6781 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6841 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6901 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6961 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     7021 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     7081 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     7141 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7201 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7261 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7321 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7381 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7441 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7501 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7561 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7621 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7681 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7741 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7801 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7861 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7921 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7981 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8041 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8101 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8161 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8221 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8281 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8341 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8401 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8461 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8521 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8581 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     8641 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgct
     8701 agcttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8761 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8821 tactagtgat tatcggatca actttgtata gaaaagttg
//
