LOCUS       sequence-318223-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector encoding a HRV 3C-cleavable 
            His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcccccttgg gagtcctcct gaaaatgatc cataatgccc tgcgcttcat tcccgtcacc
       61 tctgtggtgt tgctttacca ccgcgtccat cctgaggaag tcaccttcca cctctacctg
      121 atcccaagtg actgctccat tcggaaggcc atagatgatc tagaaatgaa attccagttt
      181 gtgcgaatcc acaagccacc cccgctgacc ccactttata tgggctgtcg ttacactgtg
      241 tctgggtctg gttcagggat gctggaaata ctccccaagg aactggagct ctgctatcga
      301 agccctggag aagaccagct gttctcggag ttctacgttg gccacttggg atcagggatc
      361 aggctgcaag tgaaagacaa gaaagatgag actctggtgt gggaggcctt ggtgaaacca
      421 ggagatctca tgcctgcaac tactctgatc cctccagccc gcatagccgt accttcacct
      481 ctggatgccc cgcagttgct gcactttgtg gaccagtatc gagagcagct gatagcccga
      541 gtgacatcgg tggaggttgt cttggacaaa ctgcatggac aggtgctgag ccaggagcag
      601 tacgagaggg tgctggctga gaacacgagg cccagccaga tgcggaagct gttcagcttg
      661 agccagtcct gggaccggaa gtgcaaagat ggactcgccc aagccctgaa ggagacccat
      721 cctcacctca ttatggaact ctgggagaag ggcagcaaaa agggactcct gccactcagc
      781 agctga
//
