LOCUS       sequence-318345-       11506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0526-sgRNA500
FEATURES             Location/Qualifiers
     source          1..11506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1434..1448,1449..1454,1455..1471,1472..1478)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1454..1470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1486..1502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1521..1532
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1539..1565,1566..1568,1569..4055,4056..4058,
                     4059..5645)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5815..6606
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6729..7317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7218..7237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        11422..11497
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgcgccaaat aatgcccgtg tccctgttga atagggacac ggttttttaa caattttttt
       61 attcctaggc cttagtggcc ggtacccggg gatccttatt actggccgct ttctagcctg
      121 gatacttgtc tggctagttg cattagagag tcagctagag ccctagggct agctgtttgt
      181 gtattcggag ccagtgtagt ttttcttttt actaacgata cgggtaataa ttgattgcct
      241 tttacagcct gaccttgaga caattgcaaa agtctgtcca cagaggtttg acctaataat
      301 ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc aactagaatc ttctgtatca
      361 tcataaccca caactgaaat atcagcccca actctaagtc cactttcagt aatagctcgc
      421 atagctccta aagccatttg atcattagca actaacatag cagtagggac aatgccttca
      481 tttaacattt gcattgtttg ctgaaatcct gacatagcag accaatctcc ttctctttct
      541 gctattggtt gtatttgatt tcttgttaaa tacttgtgcc atcctgctaa tctcaatctg
      601 gcacttactg aacttaaggg acctgcaagt agtgcaattt gttgatggcc taatgcaaca
      661 aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa aaattataga gtttattggg
      721 gtttggtccg acacatccaa aaacaatgct ggaacattag tacatgctgc ttcaactgca
      781 atcgcatctt ggtcatcaag aggataatta attataagtc cactaacacg ttgagctaat
      841 aaattatgta ctgctgcctt acaagcctct acacctgatc tctcaaccat tgatacaaca
      901 acgcttgcac caagttgatc agctctcgat ttaattgcgg ctacaatttg actaggcgca
      961 tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg attgtttccc cgcaagttgc
     1021 tgagcaacgc gatttggtat ataattcaac tctgccatag ctgcttcaac cttctctctt
     1081 gtttttgcag atacatgact tgcttggttc acaactctcg aaaccgtttg gtagctaacc
     1141 ccggcatatt cagcaacatc atacaaagta acaggtttca tggatcattc ctccatgata
     1201 aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt aaaaagaata gcacaaaaat
     1261 taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata attattatta tttatttctt
     1321 tgattaaaat caaaagcctt taatgtattt tcttgatctt tcatgcgctt aacggtaaaa
     1381 tccaaggtat taagttcacc ttccactttt ctctctctat cttctctaga aaatcataaa
     1441 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
     1501 aatttcacac agaattcatt aaagaggaga aattacatat ggataagaaa tactcaatag
     1561 gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc
     1621 cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta
     1681 taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag
     1741 ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa
     1801 atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg
     1861 aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt
     1921 atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata
     1981 aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt
     2041 ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt
     2101 tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg
     2161 ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc
     2221 agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt
     2281 tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa
     2341 aagatactta cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg
     2401 atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag
     2461 taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac
     2521 atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata
     2581 aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta
     2641 gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg
     2701 aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg
     2761 gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag
     2821 acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa
     2881 ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga
     2941 agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag
     3001 ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac
     3061 taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca
     3121 aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca
     3181 ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt
     3241 atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta
     3301 atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg
     3361 ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag
     3421 atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga
     3481 tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta
     3541 atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt
     3601 ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca
     3661 ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag
     3721 ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg
     3781 tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc
     3841 agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta
     3901 tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa
     3961 atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat
     4021 tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa agtttcctta
     4081 aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg
     4141 ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa
     4201 acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt
     4261 tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca
     4321 ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata
     4381 aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa
     4441 aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt
     4501 atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt
     4561 ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag
     4621 aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa
     4681 cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg
     4741 aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt
     4801 ccatgcccca agtcaatatt gtcaagaaaa cagaagtcca gacaggcgga ttctccaagg
     4861 agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc
     4921 caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta
     4981 aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa
     5041 ttctggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata
     5101 aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa
     5161 acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc
     5221 tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta
     5281 gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg
     5341 agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag
     5401 ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa
     5461 atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg
     5521 atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta
     5581 tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg
     5641 actaataaaa gcttgatcag atctcagctt ttttttgaag tgcctggcag taagttgaaa
     5701 aaatttgaaa taaacttgga ctatgttaat aaaaaaatac aaattaacga acaaaaagat
     5761 ttgaattatt atataattta gttaaaagca attttaaatg agggaggttt ccatatgagg
     5821 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     5881 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     5941 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     6001 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     6061 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     6121 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     6181 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     6241 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     6301 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     6361 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     6421 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     6481 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     6541 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     6601 aaataatgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcatgacc
     6661 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6841 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7381 tttcctgcac tagatccccg gcaagttcat ccttagggtt ttcatatctc ttaaaaagtc
     7441 aatttctgct tgattaattt cttgtggatt attgtttttg cggtttttca ttttttatct
     7501 ccttgagtat tgaatttttt cttatatata aatatatagc aaaaactatt tttgccaact
     7561 tttttacaaa aaatttttta ttaaaacact tagggctttg ctaaattctc ttttaaaaga
     7621 acttagtaaa gccctatgga tttaagaact gattatactt acgtagtaaa aatactacag
     7681 atattaatag tatagaccat atcaaaatca taatcaattt atatttatct aaaattgtag
     7741 tccatacaaa atacaaaaaa attaggtttt tctaaattct attttaaaag aacttagtaa
     7801 cacctaatct ctaaattact tacaaaattc taaaattaga tttttagttc ttcatatttc
     7861 tttaaaagtt ttttaagaaa atctttttga ttttcaaaaa tttcatgcat cataaaacta
     7921 gtaaatttgg attttttttt gtaaaaatca taactttctt gggttttaag ttgaaatctt
     7981 aatggtttta ttggattttg cctagatttt tttaacgccg gactttcttt atcttttata
     8041 aaattataag aatttttaat accattttct attaaataat cttcttttat aagcccctct
     8101 tgcagtgcag ttgctaatct taaatactta tacgcttgag tttttgctac atcaaagtct
     8161 tttataaaat caagaaaagt tttataacca tcaaatttat aatattgatt atcttttatt
     8221 tcttttaaaa ttttaatatt ttccaccctg tggaatattt cttttttgaa attgtcattt
     8281 aacttttcct ttagtgtctt gtaataagaa attttacttt cattaatcat taaattttgt
     8341 tcaacataag tatctatctt ttttgttaaa actttaacaa cacgtttttt ttccataaaa
     8401 attttccttt atttttgcaa cattttcctt aattgatata attgttacta ttttagattt
     8461 tttaatatcc acttaagaat taatccctca ctagaatatt ttttattata gaattcatat
     8521 acatgttttt ctaaatctat aaatttatca acaattgctt tattataggg tgtatctatc
     8581 ttttcttttc ttaataatcg tctcatattc tttaaataac aacaaacact gccggtttta
     8641 aacctaaatt ctatgtaata tatttttgaa aaagtatata cattttcaac cccgtttatt
     8701 tcatacttta caaaaacatt atttattggt tttctataac catagtaaat gcctaaaaat
     8761 ttgtccccgt ctttcatagg atatagataa agtttttgaa tctctgattt atctaataat
     8821 ttttcaaaaa aaattataca ccgcttttgt ttgttactaa ctactctatg caatgccttt
     8881 aatatttttg tgtgatatat atttttatca cctttttttt ctttcagcag gaagatactt
     8941 tcttttggaa atttaatttt ttgttcattt attcttgcag ccttaagtcc gattttcata
     9001 tttaatcctt atatagcctt tctatttaat tcttttaaac tcaataaact tttttggttt
     9061 ttcattagtt ctaataattc gcaataatat gtgcaaaaca ctttattgta ttctattttc
     9121 ttttggttat tcaaatattc ttttataatt ggctttagaa gttcaatatt tacttctttt
     9181 tttagttgtt caataaggat attgaaaatg tttgttttta aatcttgata attttgttga
     9241 gaattttctt tttttctttc aattgacttc tctaatttac gctttatatt gtttaaatcg
     9301 ctatatttat gactttcaat aataaaatga ggcttaaatt tgtaattttc gtatattttt
     9361 tggaaacttg tttctaattg tttagaatta tattcatttt tttcaaattc tttttgagtg
     9421 ttgcatagaa tttcctttaa tttttcttgt ttttctttca tacaagattt agtaaaatgt
     9481 atatttttat tttttagtaa tttaatttct attattttta tgattttaag tattttgatt
     9541 atattatctt tatcaatatt taaattgaat aaaattgtaa gaaaaattga aagaattttt
     9601 ttacataaaa agttacattt attgaagtaa tttcttactt gatacttttc tatttcatta
     9661 atctttcttt cttcttttat attattttta ttatttaaac actcctttaa atctacactc
     9721 ccatttttag taaatctatc tttaaggccc cttgaaactc tattttgaaa tcttaaatct
     9781 tttttttctt gaaagtattt atagattttt tggtaacatt ctttttttga aaaattaagt
     9841 tgatagtata tttcggtccc caaatttacc cctaagtgtt tgtgataatt agttgtgact
     9901 ttaaattctt tttctaattt gtaaaggtaa ttttgcagtg tttttagttt aacgggtgtt
     9961 tgactatttc ttttaaggtt ttcattaaag taatagagta tgtttttttg tgtgtatttc
    10021 ttatatgttt tatttatgta cgctagtgtt gagataagga ctactaattt atactgtaat
    10081 ttattgtagc aagttgtggt tttactggtt ttgtttatat tagataagtt ttccatttat
    10141 agctccttga ataccggatt ttttatctat aaagtatata tatcacaatt ttgatttaaa
    10201 agtaaattct ttttttaaaa atacaaaaat tttttataaa ttatatagac catgatattg
    10261 tattaatact aattgatttt aatttaaggt tgaactaaaa taaacatagt tttgtagaaa
    10321 ataatttttc attattttgc tacttgatat tggatcgtaa aaatattagg gctttacaaa
    10381 gttcttttaa aagagaattt agtaaagccc taagtctttt aataaaaaat tttttgtaaa
    10441 aaagttggca aaaatagttt ttgctatata tttatatata agaaaaaatt caatactcaa
    10501 ggagataaaa aatggaaaac ttaaacaata atccacaaaa aattaatcaa ataaataagc
    10561 aaactaattt tattggtttt gaaaatttta tagaacttga taatcaagtt aatcaaacta
    10621 aagaaaaaag taaattaagt aagataggta agaaattacc cggaataagc agtcaagtct
    10681 agtgcggccg gaaacagcta tgacatgatt acgaattcga gctccttgtt ttcaatgata
    10741 ggttttttag gtagagactt gtaagggttt gttcctggaa tataattttt aggaattttc
    10801 atatatttta ctcctattta actagctaac ctataattct attatttttt ttgtttttat
    10861 gcaatcaata ttgatatttt gattttttat gattagaatc atcatggaag agccaaagtc
    10921 aacacttata gacaaatatg atctttctaa ccttcacgaa atagcatcag ggggagcacc
    10981 tttgtctaag gaagtaggag aggcagttgc aaagaggttt catttacccg gaattaggca
    11041 aggatatggg ttaacagaaa ccacatctgc aattttaatt acacctgaag gagatgataa
    11101 accaggagct gttgggaagg ttgttccttt ttttgaggct aaagttgttg atttagatac
    11161 tggaaagact ttaggagtta atcaaagagg cgagttatgt gtaagaggac ctatgataat
    11221 gtcaggatac gtaaataatc cagaagctac aaatgcattg atagataaag atggttggtt
    11281 acactcaggc gatatagctt attgggacga agatgaacac ttttttatag ttgatagatt
    11341 gaaatctttg attaagtata aaggttatca agtagctcca gcagaattgg aaagtatttt
    11401 attgcaacac ccagctcttc cgttttagag ctagaaatag caagttaaaa taaggctagt
    11461 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgg
//
