LOCUS       sequence-318485-        3349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX series: pBBR1-based T-DNA binary vector for plant transformation
            (omega1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-B3omega1 (GB1694)
FEATURES             Location/Qualifiers
     source          1..3349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             745..1407
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1629..2420
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(2678..2683,2684..2701,2702..2708)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    3027..3051
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    3078..3102
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      join(3322..3349,1..744)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 gcccgtggac aaccctgctg gacaagctga tggacaggct gcgcctgccc acgagcttga
       61 ccacagggat tgcccaccgg ctacccagcc ttcgaccaca tacccaccgg ctccaactgc
      121 gcggcctgcg gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc
      181 tgcggcctgc gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc
      241 gcagcgaccg cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt
      301 gggggcagtc gaagggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc
      361 gggggttcca agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac
      421 aagccccgga ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc
      481 ccgcaggggt gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac
      541 ttaaaaatca acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca
      601 atagctcatt gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat
      661 aattgttgtc gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg
      721 cacccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg cattcaagcc
      781 aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc gtgggccggg
      841 cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc gcagatgggc
      901 caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg acgttctttg
      961 cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc cgtcgtgaag
     1021 ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc gtggggccag
     1081 ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga tcacgacgac
     1141 caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct gtatccgggc
     1201 gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg cattccgggc
     1261 atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg cgggcagcag
     1321 cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt ggagccgccg
     1381 acacgggtaa cgctgccgcg ccggtagaac cgcataaccg ccaatccgat cgaaccttga
     1441 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1501 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1561 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1621 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1681 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1741 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     1801 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     1861 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     1921 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     1981 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2041 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2101 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2161 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2221 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2281 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     2341 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     2401 ccaaggtagt cggcaaataa caaaggaccg catgtcgcaa gtcggcgcgc cacactaact
     2461 aaaaacaccc taacgggtgt tttttctttt cttgttcttc caaaaaaatt tacaaccgaa
     2521 caaacaaaca aatacagaaa tttagacaat aaaaatgaat tacaaccgca tatatcctgc
     2581 catcagtcat cacaaaatca acaattcctg caggctagct cgcatccttg tccggtctcc
     2641 tgacagcgag agacgtcact cattaggcac cccaggcttt acactttatg cttccggctc
     2701 gtataatgtg tggattttga gttagccagt tcccaatccg tcctaaccgg agtacaacaa
     2761 tgattacaga tagccttgcc gtggtactac aacgtcgtga ctgggaaaac cctggcgtta
     2821 cccaacttaa tcgccttgca gcacatccac ctttcgccag ttggcgtaat agcgaagagg
     2881 cgcgtaccga tcgtccgtcg cagcagctcc gctctctgaa tggcgaatgg cgctagtaac
     2941 ccgcttcggc gggtttttgc ttttcgtctc tctcctgaga ccgtttcgac caggctacta
     3001 gtacttaatt aatctttttc tccattgttt acaccacaat atatcctgcc accagtagat
     3061 ttcccggaca tgaagccatt tacaattgaa tatatcctgc cgccgctgcc gctttgcacc
     3121 cggtggagct tgcatgttgg tttctacgca gaactgagcc ggttttgtta tcaataaaaa
     3181 aggccgcgat ttgcggcctt attgttcgtc ggaagaccac cgaactgatg atggccccct
     3241 acgggcttgc tctccgggct tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg
     3301 atatgtggac gatggccgcg agcggccacc ggctggctcg cttcgctcg
//
