LOCUS       sequence-318514-        2279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0430
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     167..185
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(189..232)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      487..544
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(568..585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(739..1327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(819..838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1510..1604)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1625..2279,1..5))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2199..2218
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
       61 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      121 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      181 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      241 ggtctcagga gggaggcgtg tcgttttagt gaggtgaaga taagataata atgttgaaga
      301 taagatatcc ttaccgctat gggtaagatt catgtaggct ttatgacccc cgccgatgac
      361 gcgggagtct ctctgccgac agtggtccca aatgctgacg taagggatga cgcacatcta
      421 tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatatat actcgagacc
      481 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
      541 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      721 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
      781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1141 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     1501 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1561 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1621 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1681 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1741 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1801 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     1861 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1921 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1981 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2041 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2101 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2161 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2221 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttc
//
