LOCUS       sequence-318565-        5469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0068
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      3489..3546
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3570..3587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3741..4329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3821..3840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4512..4606)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4627..5286)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5201..5220
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     5448..5466
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcaa
       61 atggatccca ttcgcagtcg tacgcctagt cctgctagag agcttttacc tggaccacag
      121 ccagatcgag tgcagcctac agctgatcgt gggggcgcgc cacccgctgg tggacctctc
      181 gatgggttgc cggctcgaag gacaatgtcc agaactcgtt taccttcacc tcctgcccca
      241 tcacctgctt tctccgccgg gtcttttaat gaccttttga ggcaatttga tccatcactt
      301 cttgatacaa gtttgttaga tagcatgccg gctgtaggaa ctccacacac cgcggcagct
      361 cctgctgaat gggacgaagt gcagtccgga ttgagagcag ctgatgatcc tccccctaca
      421 gttagagttg cagttacggc tgcaagaccc ccacgcgcaa agccagcgcc acgacgtaga
      481 gccgctcagc ccagtgatgc tagcccggca gcacaagttg atctcaggac tcttggttat
      541 tcacagcaac agcaggaaaa gattaaatct aaagtaaggt ccactgtggc tcaacatcat
      601 gaggctctcg taggtcacgg attcactcat gctcatatag ttgcattatc tcagcatcca
      661 gccgctctcg gtaccgtggc agtgaagtac cagcatatta ttacagcttt gcctgaagct
      721 acgcatgagg atattgttgg agttggcaag cagtggagcg gtgccagagc actcgaagca
      781 cttcttacta aagctggaga gttgagaggt ccgccactac aattggacac cgggcagttg
      841 cttaaaatcg ctaagcgggg gggagttact gccgttgagg cggtacacgc atcgagaaat
      901 gcattgactg gagcccccct gaaccttaca ccagaccaag tggtcgctat cgctagtaat
      961 atcggaggca atcaggcgtt agagacagtg caacgtctat tacccgtgct atgccaggat
     1021 catgggctca ctcccgatca ggtcgtcgcc atcgctaaca acaatggcgg taaacaagca
     1081 ttagaaaccg tccaaaggtt gctgccagtc ctctgtcagg ctcacggcct tacacctgat
     1141 caagttgtag ctattgcatc taacggaggc aagcaggcac ttgagactgt ccagaggctt
     1201 ctgccagttt tatgccaggc tcatggtctg acccctaatc aggtggttgc catcgcgtct
     1261 aatggtgggg gaaaacaagc tctcgaaact gttcagaggt tattaccagt cctttgtcaa
     1321 gctcatggcc ttactcaaga tcaagtagtt gcgatcgcca gtaactctgg aggtaagcaa
     1381 gcccttgaaa ccgtgcaaag gctcctccct gtactctgcc aggcgcatgg cctcacgcct
     1441 gctcaggtgg tagctatcgc atctaacaat ggtggtaagc aggcattgga aacagtccag
     1501 agactcttcc cagtcctgtg ccaagatcat gggttgaccc ccgatcaagt ggtgactata
     1561 gcaaataaca atggagggaa gcaggcgctg gagacagttc aacgactcct tcctgttctc
     1621 tgtcaagcac acggtttgat tccagatcag gtagtggcca ttgccaacaa taacgggggc
     1681 aagcaagcgt tagaaactgt acaacggctt cttccagtgc tatgtcaagc ccacgggttg
     1741 actccagcac aggttgtcgc tattgcttca aacataggcg gaaagcaagc actggaaacc
     1801 gtgcagcgac ttctaccagt actatgtcga gctcacggac ttacccctgc tcaagttgta
     1861 gcaattgcca ataataatgg tggcaaacag gctcttgaaa cagttcaaag gcttctccct
     1921 gtcctatgtc aggcacatgg cctgacaccc gaccaagtcg ttgccatagc cagcaatatt
     1981 ggtgggaagc aagcattgga gactgtgcaa cggttattgc ctgtcctttg ccaagatcat
     2041 ggtttgacgc ctgaccaagt tgtggcgatt gcctctaatg gaggaaagca ggcccttgag
     2101 acagtccagc ggctactccc tgtcttatgc caagaccatg gcttgacccc tgcacaagtg
     2161 gttgcgatag catcacatga tgggggtaaa caggctttgg aaacggttca gaggctactg
     2221 ccagtattgt gccaggacca tggcctaaca ctggatcaag ttgttgctat agcgtcgcac
     2281 gatggaggca aacaagcgtt ggaaaccgtt caaagattgc tgcctgtttt gtgtcaagat
     2341 cacggtctta cactagatca ggttgtggca atcgcttcta atatcggggg taagcaggcc
     2401 ttagagacag tacaaagatt actaccagtt ctgtgtcagg atcacggact gactcctgac
     2461 caggtagttg ctatcgcttc aaatggtggc gggaaacagg cgcttgagac agtacagagg
     2521 ttgcttccag ttctttgcca ggatcatgga cttactccag accaggtggt ggctattgct
     2581 agtaatggcg gtggaaaaca ggccttggaa tcaattgttg ctcagttgtc aagacctgat
     2641 cctgccctgg ctgccctaac taatgaccac cttgtggcct tagcgtgtct tggtgggcgt
     2701 ccggcattag atgccgtaaa aaagggcctt ccacacgccc ctgagttgat acgtagaatt
     2761 aatcgtcgca ttcctgaacg cacctctcat cgtgtggcag accttgcgca tgttgttaga
     2821 gttttaggtt tctttcaaag tcactcccat cctgcccagg ctttcgatga tgctatgacc
     2881 caatttggaa tgagtcggca tggcttggtc cagctattta gaagggttgg tgttactgag
     2941 tttgaggctc gttgtggcac actgccgcca gcctcacaaa gatgggacag gattttgcaa
     3001 gcgtctggta tgaagcgtgc aaagccatcc cctaccagcg ctcaaacacc ggatcaagcc
     3061 tctttacatg cctttgctga ctcccttgag agagatttag atgctccatc cccgatgcat
     3121 gaaggcgacc agactagagc ttcatcgagg aagagatccc gaagtgatcg agcagtaact
     3181 ggtccttcga cacagcagtc tttcgaggtt agagtcccag aacaacgcga tgctctacac
     3241 ttacccttgt cttggagagt caaacgccca cgtactagga taggaggcgg tttaccagat
     3301 ccgggaacac ctatcgcagc agacttggct gcatctagta ctgttatgtg ggaacaggac
     3361 gctgctcctt ttgctggtgc agctgacgat ttccctgctt ttaacgaaga agaattggca
     3421 tggctcatgg agctgcttcc acaatcgggt agtgtcgggg gtacgatctg agcttcgaga
     3481 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     3541 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3721 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     3781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4141 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     4501 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     4561 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     4621 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     4681 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     4741 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     4801 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     4861 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     4921 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     4981 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     5041 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     5101 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     5161 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     5221 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     5281 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     5341 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     5401 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5461 cgaggcaga
//
