LOCUS       sequence-318643-        3311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1827
FEATURES             Location/Qualifiers
     source          1..3311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     171..188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(347..374)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(466..552)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     601..623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     615..632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     616..632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1631..1650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1632..1650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1655..1671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1655..1671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1773..1792)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1784..2593
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1851..1870)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2740..3311,1..17)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3229..3248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
       61 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      301 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
      361 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      421 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
      481 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
      541 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
      601 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgctct
      661 tcggcgggtg gcggtggatc gggaggagga ggttcgggtg gcggaggcag tggaggtggc
      721 ggaagtggcg gcggtggttc aggaggaggc ggatccatgg tctccaaagg agaagctgtg
      781 atcaaggagt tcatgcgctt caaggttcac atggaaggaa gcatgaatgg tcacgagttc
      841 gaaatcgaag gagaagggga gggccgcccg tacgagggaa ctcagaccgc caagctgaag
      901 gtcaccaagg gaggtaagtt tgtgataatc caatttcaat tcgcaatggt catcgttttt
      961 tcaggaccac ttccattctc atgggatatt ctctccccac aattcatgta cggctcccgt
     1021 gctttcatca aacacccagc cgacattcca gattactaca agcaatcttt cccagaaggc
     1081 ttcaagtggg agcgtgtgat gaacttcgag gatggtggag cagttacagt aactcaggac
     1141 acttctctcg aggtaagttt ttactccgct tttaacaatg gttgtttgac atcatttttt
     1201 caggatggca ctttgatcta caaggtcaag ctccgtggta ccaatttccc accagatgga
     1261 ccagttatgc agaagaagac gatgggatgg gaggcttcca ccgaacgatt gtacccagaa
     1321 gatggagttc tcaagggaga catcaaaatg gcgttacgcc tcaaggacgg aggtaagttt
     1381 gatgaaacgg tttcgtctta tatacactaa tggtactttt caggacgtta cctggcggat
     1441 ttcaagacca catacaaggc aaagaagcca gttcaaatgc caggagcata taacgttgac
     1501 cgcaagcttg atattacttc ccataatgaa gactacacag ttgtagaaca atacgaacga
     1561 tccgagggac gtcattcgac cggaggaatg gacgagctgt acaagcgaag agcgccatcc
     1621 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1681 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     1741 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1801 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1861 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     1921 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1981 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2041 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2101 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2161 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2221 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2281 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     2341 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2401 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2461 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     2521 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     2581 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2641 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2701 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2821 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2881 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2941 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3001 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3061 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3121 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3181 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3241 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3301 ctcgtcaggg g
//
