LOCUS       sequence-318658-        9210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1886
FEATURES             Location/Qualifiers
     source          1..9210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(266..293)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(385..471)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     520..542
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     534..551
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     535..551
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1536..1569
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1579..1608
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3425..3748)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5666..5690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5693..6718
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5693..6718
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6719..6743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7096..7129
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7154..7174
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7196..7327
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7355..7420
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7449..7468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7450..7468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7473..7489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7473..7489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7591..7610)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7602..8411
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7669..7688)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8558..9146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9047..9066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
       61 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      181 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
      241 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      301 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      361 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      421 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      481 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      541 cgacggccag tcttaagctc gggccccaaa taatgctctt cggcgggtgg cggtggatcg
      601 ggaggaggag gttcgggtgg cggaggcagt ggaggtggcg gaagtggcgg cggtggttca
      661 ggaggaggcg gatccatggt ctccaaagga gaagctgtga tcaaggagtt catgcgcttc
      721 aaggttcaca tggaaggaag catgaatggt cacgagttcg aaatcgaagg agaaggggag
      781 ggccgcccgt acgagggaac tcagaccgcc aagctgaagg tcaccaaggg aggtaagttt
      841 gtgataatcc aatttcaatt cgcaatggtc atcgtttttt caggaccact tccattctca
      901 tgggatattc tctccccaca attcatgtac ggctcccgtg ctttcatcaa acacccagcc
      961 gacattccag attactacaa gcaatctttc ccagaaggct tcaagtggga gcgtgtgatg
     1021 aacttcgagg atggtggagc agttacagta actcaggaca cttctctcga ggtaagtttt
     1081 tactccgctt ttaacaatgg ttgtttgaca tcattttttc aggatggcac tttgatctac
     1141 aaggtcaagc tccgtggtac caatttccca ccagatggac cagttatgca gaagaagacg
     1201 atgggatggg aggcttccac cgaacgattg tacccagaag atggagttct caagggagac
     1261 atcaaaatgg cgttacgcct caaggacgga ggtaagtttg atgaaacggt ttcgtcttat
     1321 atacactaat ggtacttttc aggacgttac ctggcggatt tcaagaccac atacaaggca
     1381 aagaagccag ttcaaatgcc aggagcatat aacgttgacc gcaagcttga tattacttcc
     1441 cataatgaag actacacagt tgtagaacaa tacgaacgat ccgagggacg tcattcgacc
     1501 ggaggaatgg acgagctgta caaggtaagt ttaaaataac ttcgtataat gtatactata
     1561 cgaagttatt ttcagggaga gcaaaagctt atctccgagg aggacctcta aggatgatcg
     1621 acgccaacgt cgttgaattt tcaaatttta aatactgaat atttgttttt tttcctatta
     1681 tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc aatccaaatc
     1741 taaacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca
     1801 caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt
     1861 ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca
     1921 acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag
     1981 gaaggaaact agctgccctc gattgtctat cttccattct caccaagttt caacaacttt
     2041 attcagtcat tcttctctgg ttcatttccc aaccaatctt cccgaataaa gcccatctgt
     2101 cctttcggcc acattcctca cctacatccg tactccttat ctcccgttct tatccagtct
     2161 tttatcgtct ctgcgtctcg cacactgtct cgtcggtaat gtaaaaaaaa aacaaaaaaa
     2221 acttgaaaac ttgcggaaag atttcctaat aaaaggagac gaatccggta ggaaatcgac
     2281 aagtctctgc ttctcgaaga ctaggtgagt tacatttctt tggccgaatt attttgaaat
     2341 cctaaatttg ataatttcag agatgtctgt aaaacttgcg tgttatgtga cggcttcggt
     2401 cactgtcgcc actcttatgg tatgcttcat gaccatgtca accatctact cggaggttga
     2461 tggattcaga gagaagcttg ataccgagat gaatgtgttc agagtgagtt aaattatatt
     2521 ttgaatttta aataattttt aattttctag caatctacca atggattgtg gaaggacata
     2581 gttgtcatcg gaagatctag caagcgtgtc cgttgtcaat atgaagagac caacgctacc
     2641 ccaactccac atgctgatgg atccccatct gctccaccag gtcaaccacc agcagttcca
     2701 ccagtcttca accagccaaa gactccaaat ggagccaatg gaaatggacc aacctgcaac
     2761 tgcaatgctg ataacaagtg cccagctgga ccatccggac caaagggagt tccaggagtt
     2821 ccaggactcg acggagttcc aggacttgac ggtgttccag gagttggagc tgatgatatc
     2881 gctccacaac gcgagtctgt cggatgcttc acttgcccac aaggaccagt tggaccacca
     2941 ggagctcttg gaagaccagg accacgtgga cttccaggac caagaggaca aaatggaaac
     3001 ccaggaagag atggacaacc aggacatcca ggagagcaag gatcatccgg tcaaatcgga
     3061 aagatcggag agccaggacc accaggagag aagggacgcg acgccgagca tccaatcgga
     3121 agaccaggac caaagggacc aagaggagat caaggaccaa caggaccagc tggacagaac
     3181 ggtcttcacg gaccaccagg agagccagga accgttggac cagaaggacc atctggaaag
     3241 caaggacgtc aaggaccaga cggaacccag ggagagactg gaccagacgg aagaccagga
     3301 aaggatgccg agtactgcca gtgcccagac aagtctccac catcagaggc tgtcaacgcc
     3361 aaccgtggat acagaaatat ctaaattgtt ggtgttttct aataaaatat ttgagattca
     3421 gtgagacttt tttcttggcg gcacaataaa gtttcctctt tttgttgggt cactcaacga
     3481 acacaataca aatatttgag cgctcgcatt tttctcaacc tttcgggttt aaagcacttc
     3541 aaaatggaat tgaagagaga ttgtgaaagt acatcagaca gaatcacatt ttgatagcat
     3601 tcacttcact cagatgcaaa cttttcagtg actataaaaa gtcataaaat aataacatta
     3661 aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa gaaagaagag tgatagagaa
     3721 gaagggaatg cttgaaagga ttttgcattt atcttaatca ccgtcttcga gaagacgaac
     3781 catggcgcca gtttcagaat ccaagtttct aatatagttc atcacaatgt tgacgttggt
     3841 ccaacctccg gcttgcataa tctctgggat ggaaactcca gcgcgagcca tgtcacgggc
     3901 agctccgaca cgggcggaat gtccagacca agccaagtaa cgttgtccgg aatcatcctt
     3961 ggctccgtag atgagacggt gggtagcttc gaagattcct gaaaatttat tttcagctta
     4021 ttcttagcta atatgttgtt taaacttacc ttcaagagct cgagtcgaga gttgtgatgt
     4081 tgcactcggg gcagcaactc cgttcttgcg aacacgacag aacaggtagt tgtttggatc
     4141 atcggcgact ccggaaaccg aaatccagcg ctcaacgagc ttggtaactc caagggagag
     4201 cgctttttcg actccggcag tcgagaccag cgtttttgta cgtccgatgt ggatgagcat
     4261 tcttcctccg tctgtgcgac taatatcctt gacgcgaatg cgggcaattt cggcgatacg
     4321 gagaagtgtg ttgtaagcaa ttccgaggaa ggcgaggttt cggatgtcct ggcaacgatc
     4381 ggaattttcc ataagagaac ggacctgatc gaagtcggtt ctgaaaattt cgtcaagtct
     4441 taggattgaa tacattaata tttaaactta cctttcgaag gcgagggctt gcttggctct
     4501 ctctccagca tcaacatttt ccttgcggat acgtctcatt acgagagata cggcattaga
     4561 atctgatggg cgtggaagtc cagatcggcg atggagcata ttgagctgtc cgagatgttg
     4621 ttggatcgtc ttcaccgcca atccacgagc ttggaggtag agcaagtagt cacgaacgtc
     4681 ttctggctcg gctgggaacc acttgcggtt gttaagcttg caccaggcag cccaagaacg
     4741 gcacactgag agaagcatct tccaggtgtg ctcggagaag gcttgtctgt cacggaacat
     4801 atccatgagg ttcttgcgaa cttcatcact tgtcgcgtca actggaagtg ctggaagatt
     4861 ttgatgaaca gtgagaagat ttgacatatt ttcgaagttt tttagatgca ctagaacaaa
     4921 gcgtgttggc ttcctctgag cccgctttcc ttatataccc gcattctgca gccttacaga
     4981 atgttctaga aggtcctaga tgcattcgtt tgaaaatact cccggtgggt gcaaagagac
     5041 gcagacggaa aatgtatctg ggtctcttta ttgtgtacac tacttttcca tgtaccgaat
     5101 gtgagtcgcc ctccttttgc aacaagcagc tcgaatgttc tagaaaaagg tggaaaatag
     5161 tataaatacc gttgaaaata aataccgaac aacatttgct ctaattgtga aattagaaat
     5221 cttcaaacta taatcatgtc actttaccac tatttccgtc cagctcaacg ttccgttttt
     5281 ggtgatcatt tttgctttcg tcgtaaatct acacacgcgt ctcttccgtg cgagagtcca
     5341 agccagcagc caaattcgtt gactgagtat tcaacgttta tacgttgtcg gcaacgagaa
     5401 ataggaaaat gcatcgggaa atgttctttt ttcgattttt tccaaggttt tgacaaattt
     5461 taccacgaat tttgctatgt tttcaattaa aaaatatgtt attcaactgt ttctatgagg
     5521 aaaataaggc tttgcatgta attttcttat tcagcataat ttttaattaa tttgaatttt
     5581 ctgtcctaac gtttattttg ttttcttggt tatgactgat ctgaaattaa tttttgaatt
     5641 ttaaggtaat atgtcaggcg gtgccgcaag tttgtacaaa aaagcaggct ccatgaaaaa
     5701 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     5761 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     5821 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     5881 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     5941 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     6001 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     6061 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     6121 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     6181 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     6241 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     6301 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     6361 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     6421 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     6481 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     6541 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     6601 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     6661 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagac
     6721 ccagctttct tgtacaaagt gggtccaatt actcttcaac atccctacat gctctttctc
     6781 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     6841 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     6901 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     6961 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     7021 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     7081 tgaccgcaat ttttaataac ttcgtataat gtatactata cgaagttatt ttcagggtgg
     7141 cggcagcgga ggagagaatc tgtactttca atccggatcc ggaggtggcg ggatgcctaa
     7201 agatccagcc aaacctccgg ccaaggcaca agttgtggga tggccaccgg tgagatcata
     7261 ccggaagaac gtgatggttt cctgccaaaa atcaagcggt ggcccggagg cggcggcgtt
     7321 cgtgaagggt ggtggcggtt ctggctcagg atctgactac aaagaccatg acggtgatta
     7381 taaagatcat gatatcgatt acaaggatga cgatgacaag acgcgaagag cgccatccag
     7441 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     7501 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     7561 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     7621 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     7681 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     7741 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     7801 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     7861 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     7921 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     7981 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     8041 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     8101 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     8161 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     8221 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     8281 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     8341 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     8401 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     8461 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     8521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8701 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9181 ccttttgctg gccttttgct cacatgttct
//
