LOCUS       sequence-318671-        5748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of microtubules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S, 
            Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J
  TITLE     Robust and Bright Genetically Encoded Fluorescent Markers for 
            Highlighting Structures and Compartments in Mammalian Cells
  JOURNAL   bioRxiv (2020) 160374; doi: https://doi.org/10.1101/160374
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) 160374; doi: https://doi.org/10.1101/160374"
COMMENT     Sequence Label: mNeonGreen-MapTau
FEATURES             Location/Qualifiers
     source          1..5748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      808..817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2476..2597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2513..2532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2567..2586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2604..3059)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2741..2762)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2953..2972
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3086..3190
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3192..3549
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3192..3383
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3215..3235)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3400..3535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3584..4378
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3584..4378
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3638..3657)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4248..4267
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4566..4585)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4610..4657
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4986..5574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5475..5494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca
      601 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc
      661 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc
      721 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc
      781 taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ctaccaagtc
      841 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac
      901 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt
      961 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc
     1021 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc
     1081 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg
     1141 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc
     1201 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag
     1261 tccggaagcg gcgggtccgg gagcggaagc gctgagcccc gccaggagtt cgaagtgatg
     1321 gaagatcacg ctgggacgta cgggttgggg gacaggaaag atcagggggg ctacaccatg
     1381 caccaagacc aagagggtga cacggacgct ggcctgaaag ctgaagaagc aggcattgga
     1441 gacaccccca gcctggaaga cgaagctgct ggtcacgtga cccaagctcg catggtcagt
     1501 aaaagcaaag acgggactgg aagcgatgac aaaaaagcca agggggctga tggtaaaacg
     1561 aagatcgcca caccgcgggg agcagcccct ccaggccaga agggccaggc caacgccacc
     1621 aggattccag caaaaacccc gcccgctcca aagacaccac ccagctctgg tgaacctcca
     1681 aaatcagggg atcgcagcgg ctacagcagc cccggctccc caggcactcc cggcagccgc
     1741 tcccgcaccc cgtcccttcc aaccccaccc acccgggagc ccaagaaggt ggcagtggtc
     1801 cgtactccac ccaagtcgcc gtcttccgcc aagagccgcc tgcagacagc ccccgtgccc
     1861 atgccagacc tgaagaatgt caagtccaag atcggctcca ctgagaacct gaagcaccag
     1921 ccgggaggcg ggaaggtgca aatagtctac aaaccagttg acctgagcaa ggtgacctcc
     1981 aagtgtggct cattaggcaa catccatcat aaaccaggag gtggccaggt ggaagtaaaa
     2041 tctgagaagc ttgacttcaa ggacagagtc cagtcgaaga ttgggtccct ggacaatatc
     2101 acccacgtcc ctggcggagg aaataaaaag attgaaaccc acaagctgac cttccgcgag
     2161 aacgccaaag ccaagacaga ccacggggcg gagatcgtgt acaagtcgcc agtggtgtct
     2221 ggggacacgt ctccacggca tctcagcaat gtctcctcca ccggcagcat cgacatggta
     2281 gactcgcccc agctcgccac gctagctgac gaggtgtctg cctccctggc caagcagggt
     2341 ttgtgaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     2401 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2461 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2581 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     2641 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2701 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2761 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2821 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2881 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2941 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3001 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3181 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3301 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3541 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4081 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     4141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4381 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     4561 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4621 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4681 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4741 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4801 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5641 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     5701 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
