LOCUS       sequence-318715-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mWasabi fused with an ER-targeting sequence of 
            calreticulin and the ER retrieval sequence, KDEL, under the control 
            of CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Tanida I, Furuta Y, Yamaguchi J, Kakuta S, Oliva Trejo JA, Uchiyama 
            Y
  TITLE     Two-color in-resin CLEM of Epon-embedded cells using osmium 
            resistant green and red fluorescent proteins.
  JOURNAL   Sci Rep. 2020 Dec 14;10(1):21871. doi: 10.1038/s41598-020-78879-x.
  PUBMED    33318540
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-12-14"; volume: "10(1):21871. doi"; pages: " 
            10.1038/s41598-020-78879-x"
COMMENT     Sequence Label: pCAG-mWasabi-ER
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..774
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgtcgc cgaccggtcg
       61 cacaccatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag
      121 atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc
      181 gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc
      241 ctgcccttct cctacgacat tctgaccacc gcgttcagtt acggcaacag ggccttcacc
      301 aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg
      361 gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg
      421 gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc
      481 cccgtgatgc agaaggagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc
      541 gacggcgtgc tgaagggcga cgtcaagatg aagctgctgc tggagggcgg cggccaccac
      601 cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc cgactatcac
      661 tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa ggtgaccgtt
      721 tacgagatcg ccgtggcccg caactccacc gacggcatgg acgagctgta caagagatcg
      781 tacaagaagg acgagctg
//
