LOCUS       sequence-318760-       10654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by
            human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1aGFP2AKAT7-W
FEATURES             Location/Qualifiers
     source          1..10654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             522..702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    749..874
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1371..1604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1789..1833
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2131..2248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2719..3900
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3913..3922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3919..3921,3922..3924,3925..4635)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3919..3921,3922..3924,3925..4635)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3964..3985)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4225..4244)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4572..4593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4654..4707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6615..6635)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7033..7044)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7496..7513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7502..7726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7752..7772)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7752..7770)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7791..7807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7791..7807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7804..7826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7815..7831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7839..7845,7846..7863,7864..7869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7884..7905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8022..8039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8193..8781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8273..8292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8952..9743,9744..9812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9575..9594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9813..9917)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9943..10398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10080..10101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10292..10311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10525..10547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10539..10556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10540..10556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10566..10585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10566..10584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
      541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      721 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      781 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      841 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      901 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      961 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1021 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1081 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1141 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1201 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     1261 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1321 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1381 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1441 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1501 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1561 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1621 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1681 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1741 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1801 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1861 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1921 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1981 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2041 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     2101 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2161 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2221 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2281 ttggttagta ccgggcccta cgcgtgaggg gcgcggaagc cggggcggag gaggagccaa
     2341 ccccgggctg ggctgagacc cgcagaggaa gacgctctag ggatttgtcc cggactagcg
     2401 agatggcaag gctgaggacg ggaggctgat tgagaggcga aggtacaccc taatctcaat
     2461 acaacctttg gagctaagcc agcaatggta gagggaagat tctgcacgtc ccttccaggc
     2521 ggcctccccg tcaccacccc ccccaacccg ccccgaccgg agctgagagt aattcataca
     2581 aaaggactcg cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta
     2641 gaacccagag atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt
     2701 ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaagtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 gaagcttggc gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3961 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4021 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4081 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4141 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4201 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4261 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4321 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4381 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4441 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4501 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     4561 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4621 gacgagctgt acaagggcgg cgggtccgga ggagagggca gaggaagtct cctaacatgc
     4681 ggtgacgtgg aggagaatcc tggcccaatg ccgcgaagga agaggaatgc aggcagtagt
     4741 tcagatggaa ccgaagattc cgatttttct acagatctcg agcacacaga cagttcagaa
     4801 agtgatggca catcccgacg atctgctcga gtcacccgct cctcagccag gctaagccag
     4861 agttctcaag attccagtcc tgttcgaaat ctgcagtctt ttggcactga ggagcctgct
     4921 tactctacca gaagagtgac ccgtagtcag cagcagccta ccccagtgac accgaaaaaa
     4981 taccctcttc ggcagactcg ttcatctggt tcagaaactg agcaagtggt tgatttttca
     5041 gatagagaaa ctaaaaatac agctgatcat gatgagtcac cgcctcgaac tccaactgga
     5101 aatgcgcctt cttctgagtc tgacatagac atctccagcc ccaatgtatc tcacgatgag
     5161 agcattgcca aggacatgtc cctgaaggac tcaggcagtg atctctctca tcgccccaag
     5221 cgccgtcgct tccatgaaag ctacaacttc aatatgaagt gtcctacacc aggctgtaac
     5281 tctctaggac accttacagg aaaacatgag agacatttct ccatctcagg atgcccactg
     5341 tatcataacc tctcagctga cgaatgcaag gtgagagcac agagccggga taagcagata
     5401 gaagaaagga tgctgtctca caggcaagat gacaacaaca ggcatgcaac caggcaccag
     5461 gcaccaacgg agagacagct tcgatataag gaaaaagtgg ctgaactcag gaagaaaaga
     5521 aattctggac tgagcaaaga acagaaagag aaatatatgg aacacagaca gacctatggg
     5581 aacacacggg aacctctttt agaaaacctg acaagcgagt atgacttgga tcttttccga
     5641 agagcacaag cccgggcttc agaggatttg gagaagttaa ggctgcaagg ccaaatcaca
     5701 gagggaagca acatgattaa aacaattgct tttggccgct acgagctgga cacatggtat
     5761 cattctccat atcctgaaga atatgcacgg ctgggacgtc tctatatgtg tgaattctgt
     5821 ttaaaatata tgaagagcca aacgatactc cgccggcaca tggccaaatg tgtgtggaaa
     5881 cacccacctg gtgatgagat atatcgcaaa ggttcaatct ctgtgtttga agtggatggc
     5941 aagaaaaaca agatctactg ccaaaacctg tgcctgttgg ccaaactttt tctggaccac
     6001 aagacattat attatgatgt ggagcccttc ctgttctatg ttatgacaga ggcggacaac
     6061 actggctgtc acctgattgg atatttttct aaggaaaaga attcattcct caactacaac
     6121 gtctcctgta tccttactat gcctcagtac atgagacagg gctatggcaa gatgcttatt
     6181 gatttcagtt atttgctttc caaagtcgaa gaaaaagttg gctccccaga acgtccactc
     6241 tcagatctgg ggcttataag ctatcgcagt tactggaaag aagtacttct ccgctatctc
     6301 cacaacttcc aaggcaaaga gatttctatc aaagaaatca gtcaggagac ggctgtgaat
     6361 cctgtggaca ttgtcagcac tctgcaagcc cttcagatgc tcaaatactg gaagggaaaa
     6421 cacctagttt taaagagaca ggacctgatt gatgagtgga tagccaaaga ggccaaaagg
     6481 tccaactcca ataaaaccat ggatcccagc tgcttaaaat ggacccctcc caagggcact
     6541 taaaagtaag aattcgcggc cgctcaacct ctggattaca aaatttgtga aagattgact
     6601 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6661 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6721 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6781 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6841 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6901 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6961 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7021 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7081 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7141 gcctccccgc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7201 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag tcaagatctg
     7261 ctttttgcct gtactgggtc tctctggtta gaccagagtc tctctggtta gaccagatct
     7321 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7381 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7441 tcagaccctt ttagtcagtg tggaaaatct ctagcagttt aaacccgctg atcagcctcg
     7501 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7561 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7621 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7681 tgggaagtca atagcaggca tgctggggat gcggtgggct ctatgggcgg ccgttaatta
     7741 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     7801 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata
     7861 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7921 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8101 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     9961 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    10021 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    10081 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    10141 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    10201 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    10261 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    10321 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    10381 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10441 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10501 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    10561 gcgcgtaata cgactcacta tagggcgaat tgactagtta ttaatagtaa tcaattacgg
    10621 ggtcattagt tcatagccca tatatggagt tccg
//
