LOCUS       sequence-318776-        3492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term GST uncleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 9 pCOLI_A_C_GST
FEATURES             Location/Qualifiers
     source          1..3492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      561..600
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      631..1219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1120..1139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1373..1390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1405..1544
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1470..1489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1630..1652
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1646..1837)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2074..2093
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2074..2092
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2130..2152
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2227..2880
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2835..2857
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(2971..2989)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2985..3032
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3097..3188
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta
       61 gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg
      121 gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc
      181 gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg
      241 cggcaatggc aacaacatta cgcaaactat taactggcga actacttact ctggcttcac
      301 ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg
      361 cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg
      421 gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga
      481 cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac
      541 tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
      601 cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
      781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1381 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     1441 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1501 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     1561 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1621 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1681 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     1741 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     1801 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     1861 ttctgttcat ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     1921 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     1981 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     2041 aggtgttggc accatgcatc gatcccgcga aattaatacg actcactata gggagaccac
     2101 aacggtttcc ctccggctcc ggaaataatt ttgtttaact ttaagaagga gactggatcc
     2161 gaatctagag cctgcagtct cgacaagctt ggaagcggat ctggatctgg aagcagcgcc
     2221 agcgcttccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
     2281 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
     2341 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
     2401 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
     2461 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
     2521 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
     2581 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
     2641 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
     2701 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
     2761 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
     2821 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaataa
     2881 taagtcgaga agtactagag gatgtacaac gcgtgcgcgc tgatccggct gctaacaaag
     2941 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     3001 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tcgggattag
     3061 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
     3121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3241 gccttcctgt ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca gaggaccaat
     3301 tgggggcacg agtgggatac atagaactgg acttgaatag cggtaaaatc cttgagagtt
     3361 ttcgccctga agagcgtttt ccaatgatga gcactttcaa agttctgcta tgtggagcag
     3421 tattatcccg tgtagatgcg gggcaagagc aactcggacg acgaatacac tattcgcaga
     3481 atgacttggt tg
//
