LOCUS       sequence-318783-        3263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term His-Thioredoxin 3C cleavable Amp resistant bacterial 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 18 pCOLI_A_Trx
FEATURES             Location/Qualifiers
     source          1..3263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1444..1463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1444..1462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1500..1522
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1542..1559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1560..1883
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             1896..1919
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2112..2130)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2126..2173
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2238..2329
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3194..3233
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga taccgatgaa
     1261 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     1321 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     1381 tcaatgccag cgcttcgtta atacagatgt aggtgttggc accatgcatc gatcccgcga
     1441 aattaatacg actcactata gggagaccac aacggtttcc ctccggctcc ggaaataatt
     1501 ttgtttaact ttaagaagga gatatacata tgggcagcag ccatcatcat catcatcaca
     1561 gcgataaaat tattcacctg actgacgaca gttttgacac ggatgtactc aaagcggacg
     1621 gggcgatcct cgtcgatttc tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga
     1681 ttctggatga aatcgctgac gaatatcagg gcaaactgac cgttgcaaaa ctgaacatcg
     1741 atcaaaaccc tggcactgcg ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt
     1801 tcaaaaacgg tgaagtggcg gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag
     1861 agttcctcga cgctaacctg gccagcagcg gatctctgga agttctgttc caggggcccg
     1921 gatcccggtc cgacatatga gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag
     1981 tcgcggccgc tttcgaatct agagcctgca gtctcgacaa gcttgtcgag aagtactaga
     2041 ggatgtacaa cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     2101 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     2161 gaggggtttt ttgctgaaag gaggaactat atcgggatta gcccgcctaa tgagcgggct
     2221 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2401 cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac gagtgggata
     2461 catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg aagagcgttt
     2521 tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc gtgtagatgc
     2581 ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg ttgaatactc
     2641 cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat gtagtgccgc
     2701 cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa
     2761 ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga
     2821 accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat
     2881 ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca
     2941 attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc
     3001 tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat
     3061 tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag
     3121 tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa
     3181 gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg
     3241 gtagaaaaga tcaaaggatc ttc
//
