LOCUS       sequence-318853-        8950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast cycling ARF mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-ARF5-T161A-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        101..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        405..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     559..579
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1232..1241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1238..1948
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1493..1502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1993..2581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2046..2066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2464..2475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2653..2886
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2964..3085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3001..3020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3055..3074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3125..3260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3280..3299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3281..3299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3309..3326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3309..3325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3318..3340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3467..3922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3554..3573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3764..3785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3948..4052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4053..4121,4122..4913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4271..4290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5084..5672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5573..5592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5826..5843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5960..5981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5996..6001,6002..6019,6020..6026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6034..6050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6039..6061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6058..6074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6058..6074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6093..6113
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6095..6113
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6141..6367
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6368..6548
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6595..6720
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7213..7446
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7631..7675
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7850..8350
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8269..8287
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8362..8757
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8817..8934
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      421 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      481 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      541 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      601 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagac
      661 catgggcctc accgtgtccg cgctcttttc gcggatcttc gggaagaagc agatgcggat
      721 tctcatggtt ggcttggatg cggctggcaa gaccacaatc ctgtacaaac tgaagttggg
      781 ggagattgtc accaccatcc caaccatagg cttcaatgta gaaacagtgg aatataagaa
      841 catctgtttc acagtctggg acgtgggagg ccaggacaag attcggcctc tgtggcggca
      901 ctacttccag aacactcagg gcctcatctt tgtggtggac agtaatgacc gggagcgggt
      961 ccaagaatct gctgatgaac tccagaagat gctgcaggag gacgagctgc gggacgccgt
     1021 attgctggtc ttcgccaaca agcaggacat gcccaacgcc atgcccgtga gcgagctgac
     1081 tgacaagctg gggctacagc acttacgcag ccgcacgtgg tatgtccagg ccacctgtgc
     1141 cgcccaaggc acaggtctgt acgatggtct ggactggctg tcccacgagc tgtcaaagcg
     1201 cgcggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga
     1261 taacatggcc tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt
     1321 ggactttgac atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa
     1381 cctgaagtcc accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg
     1441 gtatggcttc catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat
     1501 ggtagatggc tccggctacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct
     1561 tactgttaac taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa
     1621 ggggactggt ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg
     1681 gtgcaggtcg aagaagactt accccaacga caaaaccatc atcagtacct ttaagtggag
     1741 ttacaccact ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc
     1801 caagccaatg gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga
     1861 gctcaagcac tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt
     1921 gatgggcatg gacgagctgt acaagtaaag cggccgcgac tctagcgcta accggtggcg
     1981 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2041 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2101 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2161 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2221 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2281 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2341 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2401 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2461 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2521 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2581 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     2641 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     2701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2881 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     2941 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3121 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3181 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3241 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3301 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3901 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6061 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6121 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6181 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6241 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6301 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6361 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6541 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6601 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6661 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6721 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6781 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6841 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6901 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6961 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7021 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7081 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7141 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7201 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7261 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7321 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7381 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7441 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7501 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     7561 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     7621 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     7681 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7741 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     7801 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct tgcgcctttt
     7861 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     7921 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     7981 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     8041 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     8101 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     8161 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     8221 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     8281 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     8341 ctctccccag ggggtaccac catggccaag cctttgtctc aagaagaatc caccctcatt
     8401 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     8461 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     8521 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     8581 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     8641 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     8701 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg
     8761 cagctgcagt agtaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     8821 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8881 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     8941 tattacaggg
//
