LOCUS       sequence-318894-        4255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RT-Abi-P2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG004 (RT-Abi-P2)
FEATURES             Location/Qualifiers
     source          1..4255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2148..2195
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2415..3074)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2989..3008
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3075..3177)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3569..3586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3703..4248)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3783..3802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
       61 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc
      121 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
      181 ctaatgagtg agctaactta cattaattgc gttgcgcaac atcccgtcat catgccatca
      241 cgacgcgctg agacgctgaa aaaataaaat cagcaccacc gtcagcgcgc agtgctttcc
      301 ccgcctcgcc cgcccgcttc atgagacggt tttaatgcag ttgcattatg tcccgctcct
      361 cagtgctgcg ctccatcctg attacaaaaa ccgttatcaa aaacacatgc aaatagacgc
      421 agtcaaatgc gctaccgcct ctcgcaatac cttcaatttc atgataaaaa acatcatccc
      481 taacaagagc attatcctca tgaaaaaagt atatgaacta accagtgaag aagcactgtc
      541 atattttctt cgccatgact cctacacaac attagaatta ccggcttata ttaatttcac
      601 cacattatta aatgatatta attcatctat ccataacaaa aaaattaaaa ttgaaccaac
      661 cgccaaggag ctgatgggta aagatatcaa ttatgaggtg cttgtcagta aagatggtct
      721 atatagctgg cgtaggataa cacttatcaa tcccctttat tatgtctact tctgtagaaa
      781 aatcacagca ccagcaacct gggaaatcat aacagaaaaa ttcaaatctt ttgaatcaaa
      841 cgaccttttt acatgttcaa gcatccccgt cagaaaagac aactcgtcaa acattgctgc
      901 gtctgtaatg aattggtggg aagattttga acaaaaaagc cttgcccttg ctcttgaata
      961 cgaattcatg ttcagcactg acatctcaaa cttctaccca tcaatatata ctcatagttt
     1021 tgaatgggta ttcatatcaa aagaagaggc aaagaagaaa aaaagcaaaa ataacccagg
     1081 gggattaatt gacagccaca ttcaaatgat gatgaacaac cagacaaatg gtattccact
     1141 cggcagcaca ttgatggata catttgctga gcttatcttg ggtcaaatcg atatagaatt
     1201 aagaaaaaaa actaacgaac tcaaaataat aaactacaag gtagtacgct accgtgatga
     1261 ttaccggatc ttctctaata gcaaagatga tttagacata atatcaaaat gtttagtcaa
     1321 tgtattgggc gattttggtt tagatctaaa ctcaaaaaaa actgaactat atgaagacat
     1381 catacttcat tcgttgaaac aagctaaaaa agactacatc aaagaaaaaa gacataagtc
     1441 actccagaaa atgctctatt caatatattt attttcactt aaacatccaa actcgaaaac
     1501 aaccgttaga tatctaaatg attttcttag gaatttattt aagcgaaaga caattaaaga
     1561 taacggccaa caggttgatg ctatgcttgg tattatttca agcatcatgg caaaaaaccc
     1621 tacaacgtac ccagtaggaa cggcaatttt ctcaaaactc ctcagttttc tttatggtga
     1681 tgacacccaa aaaaaattaa caaagctaga acaactccat aaaaaactgg ataaacaacc
     1741 caatacagaa atgcttgaca tatggtttca gcgaactcaa gcaaaaataa acctagagtg
     1801 gaataaatct tataagtcag ctctatgcgt ccgtataaat gatgaactca caaaagagaa
     1861 aacattttct gtaaataatt tatggaatat tgactggatc caaggaaaag aaacaagccc
     1921 caataaagcc aaaatattat ccttgctaag aaaaacaaaa atcgttgaca cagataaatt
     1981 tgataaaatg gatgacaata taacacctga agaagttaat ctattcttta aagagcacag
     2041 caattaatat cccaaagcca tgttagtaac ataacatggc ttttttaaat cactcattat
     2101 cagttatcaa gaacgaacat aacattctat tccgaggagc gcaataacta gcataacccc
     2161 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc
     2221 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     2281 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
     2341 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
     2401 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt
     2461 aacattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
     2521 atcagcacct tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag
     2581 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2641 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2701 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2761 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2821 catatcacca gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg
     2881 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2941 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3001 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3061 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3121 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     3181 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     3241 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     3301 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     3361 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     3421 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     3481 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     3541 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     3601 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     3661 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     3721 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     3781 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     3841 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     3901 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     3961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     4021 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     4081 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     4141 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     4201 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatc
//
