LOCUS       sequence-318928-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RADAR defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG015 (RADAR)
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6663..6710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6930..7589)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7504..7523
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7590..7692)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8084..8101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8218..8763)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8298..8317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctttttgat tgaccaatac catttctttt aattatgaat aatgatgcgt caaccgatgg
       61 cgaacgggcc aaatccactc ttctacaact gcccattgtc acggtgtgga ataattaaaa
      121 attttagatt tttgagatta ttctcattac catcttgatt ttatttggtt ttgcatcaaa
      181 attcatagtt cacaagcttt tctcactcca aaaacaactg taaagggatt attgtgaaca
      241 cgatatacat accattagac agcggagagt ctgcggttct taaggatcca gataccttac
      301 ttccccgaaa tatttacgaa cagcttactc gatttattga aaaggctgtt aatgaagtac
      361 cgaagcctca cgaagcgctt aatgaaaccc gtagccataa ggctatatcg attgacggcg
      421 caagggggac aggaaaaacg tcggtgctag tgaatttgaa cgactatctg cagagtaatg
      481 ctcagcaact ggcggggaaa attcatatcc ttgatcctat cgatccgact ctacttgaag
      541 atggtgagtc gctgttcttg catattattg ttgctgccgt gcttcatgat aaagagatca
      601 aaactgccca aagcagagac ctcgataagt ccagagtgtt tacccagaag cttgagaact
      661 tggcacacgg actggagtcc gttgatttgc aacagaatca acgtggaatg gataaaattc
      721 gctccttata tggcagcaag catctggcaa attgcgttga agagttttta aaatctgcgt
      781 tggagttgat cggaaagaaa ttattgatac taccgattga tgatgtggac acttcactaa
      841 accgggcatt tgaaaatctg gaaatattgc gtcgttatct tacctctccg tatgttttgc
      901 cggtagtgag cggcgatcgc cgtttatatg atgaggtctg ctggcgagat tttcatggaa
      961 ggttgaataa ggattcagca tataatcgca agaacacata tgatattgct agagatttgg
     1021 caattgagta tcagcgtaaa attctgccgc taccgcgcag actgagtatg cccgatgtaa
     1081 gtgattactg gcagcaagat ggtatcgaag ttacgctaga taaaaatggc attcctctgc
     1141 gtaattttat ggcatggttg aaaatattta ttactggccc cgtgaatggc cttgagggta
     1201 gtgatttacc tctaccgata ccttcaatac gtgctttaac ccagttcatc aaccattgca
     1261 gggatttaat tcgtgagctt cctgaaccat tcagaaagaa agtcagtacg ctggccttac
     1321 gtcgtatgtg gcaaatgcct gatgttcctc ttgatgttct tgaaagtttt gctgaaaaac
     1381 atcgggaatt gagtaaagaa gctaagcgtg aatatgggga ggcttacaag ctattttatg
     1441 atggactaaa gaattttact gcttgggata gtaaggctta tctagaagat gataaacaat
     1501 ctgcatggct cgataggttg tgtgagtatt ttcgttttga acctaaggct ggggctgtgt
     1561 ttttaacgct tcaggcaaaa cagttctggg tctcatgggc gcagggtgac aatcgtaatc
     1621 aatcgattct tgcgactccg ctttttcaac ccttattgca taattttcgt gaatacgatg
     1681 tctttgaaag gtatgatgat ctttctgatt gggaatctca gttaagaaca aggttaccgg
     1741 agagttggtt gactgccatt aaagggcaaa aaacgctttt accctatcct gtagcagaag
     1801 cgggaattaa taccagttta aagtggaggt attgggaaga attagagaac tatgggtttg
     1861 atcctgcttt ggaaagcaag gcaaatttcc ttttgtccac gttgatgcag aggaattttt
     1921 atacaaactc taaacagtca gtcgtgataa atattggtag agtttttgaa ataattattg
     1981 ctagtcttgt ttcggattta gagttggccg acttgcagag aattagacaa cgttctccat
     2041 tttactctgc tagcgcgctt gcacctacca aaacgttaga tttggaagag gattttacga
     2101 aaaagaatac aagatttatg aataacagaa gtgaaactga cagagacatt tctgatgata
     2161 ttcttgttga tgtgccggat aaaaatgagg acgcatggaa aaaaatttgt gatgaaataa
     2221 accattggag aaagacacac aatgtggcta gtacaaactt atcaccttgg ctggtttata
     2281 aggtctttaa taaaacatat agtcaggttg ctaataatgt gtttgttccc agtggaatgc
     2341 aaaatgttga tgcggctcta aatgtttttg gtagggtttt ttatgcagtt tggtcagcat
     2401 ttggtagttt tgaaaaaggc gaattgttcg gactatccga tgtggttgct acaactaata
     2461 ttatttcggc aaaaaatttt tataatcatg ataacttccg agtgaatgtt ggaccgttta
     2521 cgcctgagca aaaccaaaat tctgacagcg atcgtgaggc atatcagcat cgcaaaatgt
     2581 atggtgaaaa aaccagagcg gtaagttatg tattagcaac tcatccgctg aaaaaatgga
     2641 tcgacgaggt attacgcact gagtttaaac aaaaacagaa tgctcagatt cagaccgaga
     2701 gaaaaatgcc gattcaggct gagaaaatta tagatatcag cccggcaaga gagtttatca
     2761 caagaaaact ttcattaaat tcacactccc ggttggttaa aacacgtata ataaaacagc
     2821 ttaagatgtt atatccaaac tacgataagg ctaaggactt cattgatgaa gttacaaacc
     2881 acttccctca gaatgatccc gcaattaata cgcttcagaa agcatttgca gaactttacc
     2941 ccgatggtga caaataatgt taactcggtc tctaagtgaa catgctgcag ggtgtttttt
     3001 cactgatgag cgtctgtcac aacgctttct agatatcctt ttatcgccac ccaaggattt
     3061 tgaaacgtgg tcatcattgc aggaggaatc tttcaagctg ctcgttaaga gcatcgatag
     3121 ccgatatcca cgcacttacc ggttaaccga cgtacgccag cttgtgggga acatatgtga
     3181 caacgggtta ctgacgagtc cgacactacc ttggctcgat gtcattgcgg atcagttact
     3241 gttgcggaat ggcgacttac tctattaccg cgaaaataag gttcaagact acgtgcgaat
     3301 agctgcggaa ctcgaccctg cccttctagt gggatggcgt cttggcgact ggcttttgca
     3361 aagcccaccg ccgcgattga cggacataac ccgtgtggtg atggcgcaga atccgttttt
     3421 tgctccacct gctaatgcag gtaaaccttt tgccgagggg cacgtacatc tcgggggagt
     3481 gacggctgga gatactattt tggatggcta tctttttgaa gagattgaac tacccaaaag
     3541 caaagatatg ttgttgtggg cgcacaaaga gcatgatgag ttaacaccgt tgataaatcg
     3601 agcaaagtct ttgcttacag ttctactttc tgccccccct caaacggttt ctgagcaaac
     3661 tcaaaatggt tttgatcagc gtaaaactgt atctgagaag tacaaggcat tacagaaccc
     3721 aatggatagc atccatcgtc tcccagactg gttattgctt gctaaaaaga atcgcggaac
     3781 tgaaagcgtc agccccggct ggtttttaaa ccaactggcg catgcctccg aaaaaaaaca
     3841 tccctcgcgc tggctgtggc tgcagctata cctttgccac tcttatcagc ttaaagacac
     3901 tcatccactg gagcgcacgg caatactctg tttttggctt acggtaaatg cgctacggcg
     3961 tcacattatt atggacggac aggggcttgc gtgttttacc gagcgttatt ttaatggtgc
     4021 tttacgtgcg ggtaagaaag ctgacagtag caatatgcgc tacctgtttg ccggtaaaga
     4081 cgatgtggcc gaagtgaaag catccccaaa ggctttcgat catgagatgg tcactggatt
     4141 ttcctcgaca ttgctgaaaa ccctcggcat tccagctgtt tttccaccgt atatttttgg
     4201 tgagcatgag attaagccag atgaacgcgt gctgcgctat attggagcac tggagcgctg
     4261 gcagttttgt gggcactttt ctcgctctaa aactgcaagt cgcggcaagc gagcaaaggc
     4321 tgatttgcag gctaactgga cagaagcgga gcgattgtta cagaaactgt acagtcataa
     4381 tggctggaat catcccgtct tcttaggggg taaacgtaac ccacattttc attttcagcc
     4441 gtcgaactgg tttcgggggc ttgatgttgc aggggatgaa aacgtactaa aaattgcagg
     4501 ctttgccccg atgctgcgct ggctacgaag tggattatat cccgtaccag aagggcttcg
     4561 cgccagtatg agttttcatt tcagtattca tgccggggag gattacgcac atccggcgtc
     4621 aggattgcgt catattgatg aaacggttcg cttctgcgaa atgcgggagg gagaccggct
     4681 aggacatgct ctggctctcg gaattgaacc tgcgctctgg gcgaaacggc atggtgaaat
     4741 gatactacct ctggatgaac atttagataa tcttgtctgg cagtggcact atgctacgct
     4801 tttatcggct tcattgcctc tcgctcaggc ggtattaccg ctgcttgagc gtagaattgc
     4861 acgctttatt gcacggtgcg aatggtgcaa aaagagacct ccgcaaatag ataacagtgt
     4921 ggtggggaaa caggcctgta gtgatgataa acctctggaa aatattacac ctgatacgct
     4981 ctaccgggcc tggctactgc ggcgtaattg ttcatatcga ctccagcaac tccacggcgg
     5041 ttcccctttg acctcgcaag agaaatgtgc gctgccggat tgggccacgc tcagcgataa
     5101 aggcaatgtg gcggcgcagc tttatcagca aagacactcg agtctccttg acgatatgcc
     5161 gccgcaactg gtagttgtgc gtgtagcgga cgaatgggga actcaggagc ttattggctt
     5221 gggaaatcct ggtaaactgc gtcagcaggc tcttgacggt aaagatatcc tccaagacat
     5281 tgatacgccg gtagagctgc aatttatgca tgctttacag gactatttgc tagatcacta
     5341 tgatcgtaaa gggttaatta tagaaaccaa cccaacatca aacgtatata tcgcgcgatt
     5401 caaaaagcac gtagagcatc ctatttttcg ttggaatcct ccggatgaag aactgttgaa
     5461 accaggcgct gaatttaatc gttatggatt gcgccgtggg ccagtcaggg ttctggtcaa
     5521 tactgacgat ccagggatta tgcctacgac attacggacg gaatttttac tactgcgaga
     5581 ggctgcgatt gagcgtggtg tcagccgaac gatggcagaa tattggctgg aaaggctgcg
     5641 cctgtacggg ctggaacagt ttcagcgtaa tcatttaaat gtatttgaag ttattgaata
     5701 gaggatttta tcgtgagtgg tacattccct tacttgcaat atacggatgt caatgggcta
     5761 caacctaagc tcaaagaaga gttgaaaaat ttacggagaa aagagtattt gtcctactgg
     5821 cctcgttttc tgatacgtag aatttcgctt tatgctcttc cattcctcat gttcttcact
     5881 tttttctttt gtctgagtct gacgaagaaa gttggggcag aggaagtgac taatattctt
     5941 ggaaccgtga gtatatcctt cagtagttgc ctgctgctgg ggattattat ttctggtgtc
     6001 gtgttactct tgcagtggac gtgcttcaac tgtaaataca gtccgcagga tacgaatgga
     6061 gttgttgggg ctcgtaagtt aaattataaa ttacttgctc atgttgtatt tgttattgca
     6121 tgcgtgcttt tatttgtttt tatttattgc accaataata aagtgtttta tggttttatc
     6181 gtgtttcttg gtttgacatt attaccattg gtaattgacc gtaccttggg ggtgactcgt
     6241 caaaatgaac gtcacaaact ctatatcaga aggttagagc gcctcgatga attgaatatt
     6301 ctccgggaga aaatgaatat taaattcgaa gaatcccatt tcatcgagta tatgaagctt
     6361 gttgatgaag ctgatcacgg aaaaaaccag gatacagtaa gcgatacatc ctattttatg
     6421 acgttgatag aaaataagct aaaagtgtaa tcggttttaa tatgatgctg tataaaaaac
     6481 tacgcaattg cgtggttttt tgtcggacta tgagggcaag gttgccctaa aacagaggtt
     6541 aaacgttggg atgtgattta ttgcacatca tgccgtgccc atccagtaga atccggttcg
     6601 aaatgtgtat aggattgtgt atatgtttct gttcggtctc ggattcttat acaccgcaat
     6661 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc
     6721 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     6781 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc
     6841 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     6901 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcaatac
     6961 tgttgtaatt catttaacat tctgccgaca tggaagccat cacagacggc atgatgaacc
     7021 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catagtgaaa
     7081 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     7141 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     7201 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     7261 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     7321 gggtgaacac tatcccatat caccagctca ccgtctttca tcgccatacg gaactctgga
     7381 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     7441 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     7501 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     7561 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     7621 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     7681 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     7741 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     7801 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     7861 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     7921 gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat
     7981 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     8041 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     8101 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     8161 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     8221 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     8341 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     8401 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     8461 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8521 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     8581 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     8641 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     8701 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     8761 caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct
     8821 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
     8881 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     8941 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgcatg tggttagtta
     9001 tcacagcact aacctatttt cga
//
