LOCUS       sequence-319115-       11354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitous expression of MCP-sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 11354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UMsfG
FEATURES             Location/Qualifiers
     source          1..11354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1739..1741,1742..1744,1745..2458)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1784..1805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2392..2413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3092..3108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3092..3108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3105..3127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3116..3132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3140..3146,3147..3164,3165..3170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3185..3206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3397..4073
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4010..4030
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4140..5165
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5206..5380
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5458..5482
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5907..6701
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6788..7376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7277..7296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7530..7547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7562..7702
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7628..7647)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7788..7810
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8046..8240
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8306..9379)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9808..10437)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccagga tgatttctgg tttaaacaag cttcggctgg atcttatgac gaccgttaga
      721 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
      781 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
      841 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
      901 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
      961 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     1021 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     1081 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     1141 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     1201 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     1261 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     1321 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagatgc atcacctggc
     1381 ttctaacttt actcagttcg ttctcgtcga caatggcgga actggcgacg tgactgtcgc
     1441 cccaagcaac ttcgctaacg ggatcgctga atggatcagc tctaactcgc gttcacaggc
     1501 ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat acaccatcaa
     1561 agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat atggaactaa ccattccaat
     1621 tttcgccacg aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga
     1681 tggaaacccg attccctcag caatcgcagc aaactccggc atctacgcgg ccactagtat
     1741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1801 cgacgtaaac ggccacaagt tcagcgtgag aggcgagggc gagggcgatg ccaccaacgg
     1861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcttt
     2041 caaggacgac ggcacttaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2161 gctggagtac aacttcaaca gccacaacgt ctatatcact gccgacaagc agaagaacgg
     2221 catcaaggcc aacttcaaga tccgccacaa cgttgaggac ggcagcgtgc agctcgccga
     2281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2341 cctgagcacc cagtccgttc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     2461 tttttgtgat ctgatgataa gtggttggtt cgtgtctcat gcacttggga ggtgatctat
     2521 ttcacctggt gtagtttgtg tttccgtcag ttggaaaaac ttatccctat cgatttcgtt
     2581 ttcattttct gcttttcttt tatgtacctt cgtttgggct tgtaacgggc ctttgtattt
     2641 caactctcaa taataatcca agtgcatgtt aaacaatttg tcatctgttt cggctttgat
     2701 atactactgg tgaagatggg ccgtactact gcatcacaac gaaaaataat aataagatga
     2761 aaaacttgaa gtggaaaaaa aaaaaaactt gaatgttcac tactactcat tgaccataat
     2821 gtttaacata catagctcaa tagtattttt gtgaatatgg caacacaaac agtccaaaac
     2881 aattgtctct tactatacca aaccaagggc gccgcttgtt tgccactctt tgtgtgcaat
     2941 agtgtgatta ccacatctcc acattcaata tattccctga attatctgac gattttgatg
     3001 gctcactgtt ttcccaagtc tggtacccca cctacatggg gatcctaact cgagctgcag
     3061 gcttcggtac cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     3121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3301 gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa
     3361 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     3421 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     3481 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     3541 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3601 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc
     3661 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
     3721 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     3781 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
     3841 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
     3901 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     3961 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     4021 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
     4081 agtctctctc tacaaatcta tctctctcga gctttcgcag atccgggggg caatgagata
     4141 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     4201 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     4261 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     4321 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     4381 gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt gtcacgttgc
     4441 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
     4501 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     4561 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     4621 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     4681 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     4741 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     4801 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     4861 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     4921 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4981 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     5041 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     5101 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga
     5161 aatagagtag atgccgaccg ggatctgtcg atcgacaagc tcgagtttct ccataataat
     5221 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     5281 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     5341 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt
     5401 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     5461 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     5521 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     5581 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     5641 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     5701 agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac
     5761 gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
     5821 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
     5881 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     5941 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     6001 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     6061 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     6121 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     6181 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     6241 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     6301 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     6361 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     6421 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     6481 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     6541 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     6601 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     6661 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca
     6721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6901 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7561 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     7621 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     7681 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     7741 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7801 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     7861 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     7921 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     7981 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     8041 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     8101 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     8161 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     8221 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     8281 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     8341 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     8401 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     8461 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     8521 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     8581 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     8641 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     8701 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     8761 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
     8821 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
     8881 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
     8941 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
     9001 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
     9061 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
     9121 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
     9181 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
     9241 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
     9301 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
     9361 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
     9421 ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
     9481 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
     9541 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
     9601 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
     9661 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
     9721 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
     9781 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
     9841 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
     9901 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
     9961 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    10021 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    10081 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    10141 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    10201 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    10261 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    10321 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    10381 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    10441 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    10501 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    10561 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    10621 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    10681 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    10741 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    10801 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    10861 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    10921 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    10981 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    11041 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    11101 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    11161 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    11221 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    11281 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    11341 cggcccgttg gttg
//
