LOCUS       sequence-319139-       11243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitously expressed nuclear localized tagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UtB2N7
FEATURES             Location/Qualifiers
     source          1..11243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1373..2083
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(2981..2997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2981..2997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2994..3016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3005..3021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3029..3035,3036..3053,3054..3059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3074..3095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3286..3962
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3899..3919
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4029..5054
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5095..5269
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5347..5371
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5796..6590
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6677..7265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7166..7185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7419..7436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7451..7591
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7517..7536)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7677..7699
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7935..8129
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8195..9268)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9697..10326)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccagga tgatttctgg tttaaacaag cttcggctgg atcttatgac gaccgttaga
      721 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
      781 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
      841 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
      901 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
      961 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     1021 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     1081 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     1141 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     1201 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     1261 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     1321 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacaggtta acatggtgtc
     1381 taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     1441 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     1501 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1561 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     1621 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     1681 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     1741 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     1801 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     1861 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag
     1921 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     1981 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     2041 cagatactgc gacctcccta gcaaactggg gcacaagctt aatgttaaca ttgctgcagc
     2101 ggccgaattc aagcgtgaag agcaagcaag gaaagctaag gtgaacaatg agaaaaagac
     2161 ggaaatagtg aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc tgaaaagaaa
     2221 ggactctgag gatggaaacg agggtgagga agaagaagct tcttcgaaac cgaaaaagcc
     2281 aaaagttgct ctttctcatc ttcaggacat tgacgacaca gaagctgatc aagaagaaga
     2341 gtaaaacgct ttttgtgatc tgatgataag tggttggttc gtgtctcatg cacttgggag
     2401 gtgatctatt tcacctggtg tagtttgtgt ttccgtcagt tggaaaaact tatccctatc
     2461 gatttcgttt tcattttctg cttttctttt atgtaccttc gtttgggctt gtaacgggcc
     2521 tttgtatttc aactctcaat aataatccaa gtgcatgtta aacaatttgt catctgtttc
     2581 ggctttgata tactactggt gaagatgggc cgtactactg catcacaacg aaaaataata
     2641 ataagatgaa aaacttgaag tggaaaaaaa aaaaaacttg aatgttcact actactcatt
     2701 gaccataatg tttaacatac atagctcaat agtatttttg tgaatatggc aacacaaaca
     2761 gtccaaaaca attgtctctt actataccaa accaagggcg ccgcttgttt gccactcttt
     2821 gtgtgcaata gtgtgattac cacatctcca cattcaatat attccctgaa ttatctgacg
     2881 attttgatgg ctcactgttt tcccaagtct ggtaccccac ctacatgggg atcctaactc
     2941 gagctgcagg cttcggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt
     3001 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3061 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3121 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3181 gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta
     3241 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
     3301 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
     3361 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
     3421 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     3481 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca
     3541 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     3601 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     3661 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     3721 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     3781 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     3841 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     3901 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
     3961 gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tccggggggc
     4021 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     4081 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     4141 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     4201 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     4261 ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt tcacagggtg
     4321 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg
     4381 ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     4441 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     4501 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     4561 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     4621 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     4681 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     4741 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     4801 gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     4861 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4921 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4981 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     5041 gggcaaagaa atagagtaga tgccgaccgg gatctgtcga tcgacaagct cgagtttctc
     5101 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     5161 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     5221 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa
     5281 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     5341 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     5401 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     5461 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     5521 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     5581 cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact
     5641 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     5701 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     5761 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     5821 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     5881 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     5941 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     6001 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     6061 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     6121 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     6181 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     6241 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     6301 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     6361 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     6421 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     6481 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     6541 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat
     6601 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6661 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6721 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6781 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6841 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6901 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6961 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7021 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7081 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7141 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7201 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7261 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7321 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7381 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7441 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7501 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     7561 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     7621 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     7681 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     7741 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     7801 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     7861 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     7921 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     7981 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     8041 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     8101 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     8161 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     8221 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     8281 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     8341 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     8401 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     8461 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     8521 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     8581 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     8641 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     8701 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     8761 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     8821 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     8881 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     8941 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     9001 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     9061 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     9121 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     9181 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     9241 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     9301 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     9361 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     9421 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     9481 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     9541 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     9601 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     9661 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     9721 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     9781 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     9841 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     9901 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     9961 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    10021 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    10081 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    10141 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    10201 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    10261 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    10321 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    10381 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    10441 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    10501 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    10561 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    10621 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    10681 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    10741 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    10801 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    10861 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    10921 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    10981 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    11041 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    11101 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    11161 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    11221 cgtgcagttc ggcccgttgg ttg
//
