LOCUS       sequence-319260-        9088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Shin HR, See JE, Kweon J, Kim HS, Sung GJ, Park S, Jang AH, Jang G, 
            Choi KC, Kim I, Kim JS, Kim Y
  TITLE     Small-molecule inhibitors of histone deacetylase improve 
            CRISPR-based adenine base editing.
  JOURNAL   Nucleic Acids Res. 2021 Feb 26;49(4):2390-2399. doi: 
            10.1093/nar/gkab052.
  PUBMED    33544854
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-02-26- 26"; volume: "49"; issue: 
            "4"; pages: "2390-2399"
COMMENT     Sequence Label: EFS-ABE7.10-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..9088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(39..539,540..635,636..1133,1134..1229)
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.10)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             join(1230..4628,4629..4631,4632..5228,5229,5230..5232,
                     5233..5234,5235..5237,5238..5330)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1230..1253,1254..1256,1257..5330)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5331..5387
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5388..5390,5391..5393,5394..6104)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5388..5390,5391..5393,5394..6104)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5433..5454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5694..5713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6041..6062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6117..6137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6146..6163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6186..6203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6192..6416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6487..6503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6487..6503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6500..6522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6511..6527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6535..6541,6542..6559,6560..6565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6580..6601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6718..6735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6889..7477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6969..6988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7648..8439,8440..8508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8271..8290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8509..8613)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8692..8711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8877..9088
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ggccgctaat acgactcact atagggagag ccgccaccat gtccgaagtc gagttttccc
       61 atgagtactg gatgagacac gcattgactc tcgcaaagag ggcttgggat gaacgcgagg
      121 tgcccgtggg ggcagtactc gtgcataaca atcgcgtaat cggcgaaggt tggaataggc
      181 cgatcggacg ccacgacccc actgcacatg cggaaatcat ggcccttcga cagggagggc
      241 ttgtgatgca gaattatcga cttatcgatg cgacgctgta cgtcacgctt gaaccttgcg
      301 taatgtgcgc gggagctatg attcactccc gcattggacg agttgtattc ggtgcccgcg
      361 acgccaagac gggtgccgca ggttcactga tggacgtgct gcatcaccca ggcatgaacc
      421 accgggtaga aatcacagaa ggcatattgg cggacgaatg tgcggcgctg ttgtccgact
      481 tttttcgcat gcggaggcag gagatcaagg cccagaaaaa agcacaatcc tctactgact
      541 ctggtggttc ttctggtggt tctagcggca gcgagactcc cgggacctca gagtccgcca
      601 cacccgaaag ttctggtggt tcttctggtg gttcttccga agtcgagttt tcccatgagt
      661 actggatgag acacgcattg actctcgcaa agagggctcg agatgaacgc gaggtgcccg
      721 tgggggcagt actcgtgctc aacaatcgcg taatcggcga aggttggaat agggcaatcg
      781 gactccacga ccccactgca catgcggaaa tcatggccct tcgacaggga gggcttgtga
      841 tgcagaatta tcgacttatc gatgcgacgc tgtacgtcac gtttgaacct tgcgtaatgt
      901 gcgcgggagc tatgattcac tcccgcattg gacgagttgt attcggtgtt cgcaacgcca
      961 agacgggtgc cgcaggttca ctgatggacg tgctgcatta cccaggcatg aaccaccggg
     1021 tagaaatcac agaaggcata ttggcggacg aatgtgcggc gctgttgtgt tacttttttc
     1081 gcatgcccag gcaggtcttt aacgcccaga aaaaagcaca atcctctact gactctggtg
     1141 gttcttctgg tggttctagc ggcagcgaga ctcccgggac ctcagagtcc gccacacccg
     1201 aaagttctgg tggttcttct ggtggttctg ataaaaagta ttctattggt ttagccatcg
     1261 gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat
     1321 ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc
     1381 tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt
     1441 atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca
     1501 aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga
     1561 aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt
     1621 acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga
     1681 ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg
     1741 gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct
     1801 ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg aaggctattc
     1861 ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa ttacccggag
     1921 agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg acaccaaatt
     1981 ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag gacacgtacg
     2041 atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac ttatttttgg
     2101 ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt aatactgaga
     2161 ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat caccaagact
     2221 tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag gaaatattct
     2281 ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt caagaggaat
     2341 tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag ttgcttgtaa
     2401 aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt agcattccac
     2461 atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat ttttatccgt
     2521 tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata ccttactatg
     2581 tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag tccgaagaaa
     2641 cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct caatcgttca
     2701 tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg cctaagcaca
     2761 gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag tatgtcactg
     2821 agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata gtagatctgt
     2881 tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac tttaagaaaa
     2941 ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat gcgtcacttg
     3001 gtacgtatca tgacctccta aagataatta aagataagga cttcctggat aacgaagaga
     3061 atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat cgggaaatga
     3121 ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg aaacagttaa
     3181 agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac gggataagag
     3241 acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc gccaatagga
     3301 actttatgca gctgatccat gatgactctt taaccttcaa agaggatata caaaaggcac
     3361 aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct ggttcgccag
     3421 ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt aaggtcatgg
     3481 gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa acgactcaga
     3541 aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt aaagaactgg
     3601 gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac gagaaacttt
     3661 acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg gacataaacc
     3721 gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag gacgattcaa
     3781 tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac aatgttccaa
     3841 gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat gcgaaactga
     3901 taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg tctgaacttg
     3961 acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca aagcatgttg
     4021 cacagatact agattcccga atgaatacga aatacgacga gaacgataag ctgattcggg
     4081 aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag gattttcaat
     4141 tctataaagt tagggagata aataactacc accatgcgca cgacgcttat cttaatgccg
     4201 tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt gtgtatggtg
     4261 attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag ataggcaagg
     4321 ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg gaaatcactc
     4381 tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag acaggtgaaa
     4441 tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc atgccccaag
     4501 tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa tcgattcttc
     4561 caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg aaaaagtacg
     4621 gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa gttgagaagg
     4681 gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt atggagcgct
     4741 cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag gaagtaaaaa
     4801 aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat ggccgaaaac
     4861 ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta ccgtctaaat
     4921 acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca cctgaagata
     4981 acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa atcatagagc
     5041 aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac aaagtattaa
     5101 gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat attatccatt
     5161 tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac acaacgatag
     5221 atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt caccaatcca
     5281 tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac gctactaact
     5341 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gtgagcaagg
     5401 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5461 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5521 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5581 tgacctatgg agtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5641 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5701 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5761 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5821 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     5881 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5941 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     6001 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     6061 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtctggt ggttctccca
     6121 agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg
     6181 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6241 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6301 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6361 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6421 tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta
     6481 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6541 acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt
     6601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6661 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6721 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6781 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6841 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6901 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6961 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7021 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7081 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7141 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7201 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7261 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7321 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7381 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7441 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7501 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7621 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8521 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8641 cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg
     8701 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     8761 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     8821 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatagggc
     8881 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg
     8941 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     9001 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     9061 tcgcaacggg tttgccgcca gaacacag
//
