LOCUS       sequence-319263-        3219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Berlew EE, Kuznetsov IA, Yamada K, Bugaj LJ, Boerckel JD, Chow BY
  TITLE     Single-Component Optogenetic Tools for Inducible RhoA GTPase 
            Signaling.
  JOURNAL   Adv Biol. 2021 Sep;5(9):e2100810. doi: 10.1002/adbi.202100810.
  PUBMED    34288599
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Biol.";
            date: "2021-09"; volume: "5(9):e2100810. doi"; pages: " 
            10.1002/adbi.202100810"
COMMENT     Sequence Label: opto-RhoGEF-mCherry_pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..3219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2506..3216
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2651..2669)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2919..2938
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggccacag acgcaatcga ccattactac cagaacagag acggcccccc ttccaggagc
       61 tttagcgacg aatacgactc atacaataag acagcccctc tgccacctct gagcgcctgc
      121 ccagagcgcg cccagacccc taacagcaag agaatcccca tcacacccaa ttccgccaag
      181 agcacctccg agtctcctcg gagaatcaca ttctctcgcg acgcaggcag ctccaccaag
      241 ggcagcaccc agacaagccc atccaaccag ggctccgtgt ctagccggaa tggctctaac
      301 ggaaatggcc tgggcctgtc ctctgccggc aacttcgccg atttctttag ctccgaggtg
      361 ttttatatcg tgctgcacaa tcccaccaca gcccacagat tcctgaagtt ttgccagagc
      421 agggcctgtt ccgagaacct ggagtttctg cagaagatcg acgagtacaa taggctgatc
      481 gatgaggcca cacgcctgct gtctaccatc tacagcacat acgtgagcag cgaggcccct
      541 aagcagatca acatctctaa tagccacgcc aggcgcctga gccacgacat caagggagca
      601 acccacaacg tgctgcccgg cctggaggat atcttcaatg gctcccagga gcagatcgag
      661 aagctgctgg cctctgacct gtatcctaga tttgtgaagc accaggtgac cgcatccgcc
      721 acaatggccc tggcaaacga caaggagagg tacccaggcc tgggcgattg cttctgtctg
      781 acagacccac ggagagccga taatcccatc gtgttcgcaa gcgacggatt cgtgagcgtg
      841 accggctatt cccgctctga tatcatccca aggaactgcc gcttcctgca gggcagcttt
      901 accgaccggc aggccacaaa gaggctgcgc acctccatcg agaatcgcga ggagacagtg
      961 gagctgctgc tgaactatcg gaagaatggc gatccctttt ggaacctgct gtacgtgtcc
     1021 cctctgctgg acggcaatgg cgatgtgaga ttctttctgg gcggccagat caactgttct
     1081 accacaatcc acagccggac cgacgtgctg agaatcctga gcctgaacga cgaggataca
     1141 ggctccttca tcgagggctc taataagacc cccagcgtgc ggtccaagga gtctaacaat
     1201 gcctccaact atggcaagac atccttcttt aagtctttta agaagtacaa ggccaatacc
     1261 gtggagatca gagacgaggc cggcatggag ggcgagctga tcgataggat cggcaagctg
     1321 cagttcaaga cacaggtgga ggagttttac accgcctatt ctaagtacct ggtgctgagc
     1381 tatcagccac agtcccagaa cctgatcgtg aagtacttca gccccggcat cgtggatatg
     1441 ctgaacctga atatgcctaa tggcctggtg gccccaatcg tggacaagga tatcttcaag
     1501 gtgctgacag agcactcccc ttcctctgtg ccacggacct ttaagaacgt ggtgagggaa
     1561 ggcatcaaag caggcagggc cgtgtctgtg gagacaggcc tgctgacagg catcgaggag
     1621 atcaagaagt ctcagaacat cttcagcggc accacagcca atcgggagag aggctggaag
     1681 aaggccgagg agaagtacgt gtgccacttt accccctgta aggatgagat gggcagggtc
     1741 ggctgggtgg tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg tggcggatca
     1801 ggaggagggt caatgctgac ccagagagaa attgataggc aggaagtgat taacgagctg
     1861 tttgtgaccg aggcaagcca tctgagaacc ctgagagtgc tggacctgat cttctaccag
     1921 aggatgaaga aagagaacct gatgccccgg gaggaactgg ctagactgtt tccaaacctg
     1981 cccgagctga tcgaaattca caattcctgg tgcgaagcaa tgaagaaact gcgcgaggaa
     2041 ggacctatca ttaaggagat ttctgacctg atgctggcta ggttcgatgg cccagcacgc
     2101 gaggaactgc agcaggtggc cgctcagttt tgctcatacc agagcatcgc cctggaactg
     2161 attaagacta aacagagaaa ggagtctagg ttccagctgt ttatgcagga ggctgaaagt
     2221 cacccacagt gtcggagact gcagctgcgc gacctgatca ttagcgaaat gcagcgactg
     2281 acaaagtatc ccctgctgct ggaaagcatc atcaagcaca ctgagggcgg gacctccgag
     2341 catgaaaagc tgtgcagggc ccgcgatcag tgtcgggaga tcctgaagta cgtgaacgag
     2401 gctgtcaaac agaccgaaaa tcgacatcgg ctggagggct atcagaaaag actggacgct
     2461 acagcactgg aaagggccag tggtggtgga tcaggtggag gatctatggt gagcaagggc
     2521 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2581 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2641 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2701 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2761 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2821 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2881 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2941 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     3001 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     3061 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     3121 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     3181 cactccaccg gcggcatgga cgagctgtac aagtaatga
//
