LOCUS       sequence-319285-        3739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DsRed for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3739)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD DsRed-CT (GB1183)
FEATURES             Location/Qualifiers
     source          1..3739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(99..117)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(100..117)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(135..151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(135..151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(148..170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    159..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(183..189,190..207,208..213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(366..383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(537..1125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(617..636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1296..2087,2088..2156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1919..1938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2157..2261)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2339..2794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2476..2497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2688..2707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2920..2942
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2934..2951
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2935..2951
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2958..2977
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2958..2976
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3007..3016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3011..3020
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3058..3735
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3238..3258)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3650..3673
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgagaccact catcgcccaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg
       61 ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc
      121 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      181 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      241 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      961 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac
     1441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2281 ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     2341 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2401 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     2461 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     2521 gtcaaagggc gaaaaaccgt ctatcagggc gctggcccac tacgtgaacc atcaccctaa
     2581 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2641 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2701 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2761 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     2821 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2881 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2941 cgacggccag tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg
     3001 ctcccggccg ccatggcggc cgcgggaatt cgattgggcg atgagtggtc tcgttcgatg
     3061 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc
     3121 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac
     3181 aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     3241 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     3301 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3361 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag
     3421 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagacgatg
     3481 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagaca
     3541 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac
     3601 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc
     3661 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac
     3721 cacctgttcc tgtaggctt
//
