LOCUS       sequence-31956-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector containing LacZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223_LacZ
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(122..163)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    216..240
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggc aaaccggtac
      121 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
      181 ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt
      241 cggcgcgccc acccttagtt ttttgacacc agaccaactg gtaatggtag cgaccggcgc
      301 tcagctgtaa ttccgccgat actgacgggc tccaggagtc gtctccacca atccccatat
      361 ggaaaccgtc gatattcagc catgtgcctt cttccgcgtg cagcagatgg cgatggctgg
      421 tttccatcag ttgctgttga ctgtagcggc tgatgttgaa ctggaagtcg ccgcgccact
      481 ggtgtgggcc ataattcaat tcgcgcgtcc cgcagcgcag accgttttcg ctcgggaaga
      541 cgtacggggt atacatgtct gacaatggca gatcccagcg gtcaaaacag gcggcagtaa
      601 ggcggtcggg atagttttct tgcggcccta atccgagcca gtttacccgc tctgctacct
      661 gcgccagctg gcagttcagg ccaatccgcg ccggatgcgg tgtatcgctc gccacttcaa
      721 catcaacggt aatcgccatt tgaccactac catcaatcc
//
