LOCUS       sequence-319597-       10919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10919)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ER-J-TAG (pGS730)
FEATURES             Location/Qualifiers
     source          1..10919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1570..1687
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2786..3972
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3017..3963
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3920..3940
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4062..4142
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    4974..5526
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5118..5135)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5345..5364
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5535..5930
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5940..5996
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6006..6551
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             6561..6581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6588..7176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6641..6661)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7059..7070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7562..7795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7867..7988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7904..7923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7958..7977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8090..8109
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8374..8392)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8460..8564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8565..8633,8634..9425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8783..8802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9596..10184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10085..10104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    10512..10533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10548..10553,10554..10571,10572..10578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10586..10602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10591..10613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10610..10626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10610..10626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10645..10665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10647..10665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10693..10919
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1621 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1681 aaattttatc gataccgtcg agtttactcc ctattctaga caaatgcttg tccttccttc
     1741 ctgtaacaca gccattaaac caggagcatc gcccttcccc ggccctcagg taagaggacc
     1801 aaataccgta gccgtttcca atttcagtcc tttagcgcca cctggtgcta actactctat
     1861 cacgctttta tccaataact acctttgtaa atttcctttc aaaagttctg gccgggcgcg
     1921 gtggctcacg cttgtaatcc cagcactttg tgaggggtca ggagttcgag acattaatag
     1981 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
     2041 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
     2101 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
     2161 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
     2221 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
     2281 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
     2341 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
     2401 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
     2461 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg
     2521 ggcgcgcgcc cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc
     2581 cccggcggcc ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta
     2641 gcgcggagtt taattaaaat cgcgtaccgt cgacctcgac ggatcgggag atctcgaccg
     2701 agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     2761 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     2821 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2881 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2941 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3001 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     3061 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     3121 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     3181 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     3241 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     3301 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatcgatc
     3361 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3421 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3481 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3541 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3601 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3661 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3721 agtaccgggc gccgtccagg cacctcgatt agttctcgat cgagcttttg gagtacgtcg
     3781 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3841 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3901 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3961 caggtgtcgt gaggaattag cttgaccaag cttggtaccg agctcggatc cactagtaac
     4021 ggccgccagt gtgctggaat tatcacaagg ccgccaccat gtacccatac gatgttccag
     4081 attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg
     4141 ccggcggatc aagtaagggg gaggagctgt ttacgggtgt agtacccata cttgtcgaac
     4201 tggacggtga tgttaatgga cataagttta gtgtcagagg ggaaggggaa ggggatgcaa
     4261 cgaatggaaa gctgacgctc aagttcatct gtacaactgg caaactcccg gtgccttggc
     4321 caactctcgt aaccacattg acatacggag tccagtgctt tagtaggtat ccggatcata
     4381 tgaagcagca tgatttcttt aagtcagcca tgccagaggg ttatgtccag gaacgaacta
     4441 tcagttttaa ggatgatgga acgtacaaaa ctcgagcaga agtcaagttc gaaggcgata
     4501 ctttggtaaa ccgcatagaa ctcaaaggga tcgacttcaa agaagatgga aacatactgg
     4561 gccataaact ggaatataac tttaactccc acaatgttta catcactgcc gataagcaga
     4621 agaacggtat taaggccaat tttaagatta ggcataatgt agaagatggg tcagttcaat
     4681 tggcggatca ttatcagcaa aacacgccga taggagacgg tcccgtgctt ctcccagaca
     4741 accactatct tagcacacag tctaagctga gtaaggaccc taatgagaag agggatcaca
     4801 tggtacttct ggaatttgtt acggcggcag gtatcaccca cggtatggat gaactctaca
     4861 agggtggtgg ttctggaggc ggcggatcac ctaattcaat catgattgtc cttgtcatgc
     4921 tgttgaatat cgggttggcc attctttttg ttcactttct aacttgaaaa ctaacgttac
     4981 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     5041 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     5101 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     5161 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     5221 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     5281 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     5341 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     5401 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     5461 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     5521 ataatatggc cacaatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     5581 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     5641 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     5701 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     5761 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     5821 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     5881 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ggatccggcg
     5941 caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgggat
     6001 ccggcatgga caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc
     6061 tggaactgtc tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat
     6121 ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac
     6181 tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt
     6241 tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc
     6301 tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg
     6361 ccctggccgg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg
     6421 tgcccattct gatcccctgc caccgggtgg tgcagggcga cctggacgtg gggggctacg
     6481 agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc
     6541 ctgggctggg tggcgggagc ccaaagaaga agcggaaggt gtagcggaat caacctctgg
     6601 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6661 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6721 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6781 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6841 ccaccacctg tcagctcctt tccggaactt tcgctttccc cctccctatt gccacggcgg
     6901 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6961 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     7021 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7081 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7141 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgagg
     7201 tacgccaaca gagcgagatt ccgtctcaaa gaaaaaaaaa gtaatgaaat gaataaaatg
     7261 agtcctagag ccagtaaatg tcgtaaatgt ctcagctagt caggtagtaa aaggtctcaa
     7321 ctaggcagtg gcagagcagg attcaaattc agggctgttg tgatgcctcc gcagactctg
     7381 agcgccacct ggtggtaatt tgtctgtgcc tcttctgacg ttgaagaaca gcaactaaca
     7441 cactaacacg gcatttacta tgggccagcc attgttctaa tgttaactgt ggtaccttta
     7501 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7561 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7861 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7981 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8041 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8101 aggccgaggc cggatccctt gagtggcttt catcctggag cagactttgc agtctgtgga
     8161 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     8221 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     8281 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     8341 aaggccccct tgttaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     8401 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8461 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8581 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8641 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8701 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     8821 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8881 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8941 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9061 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     9121 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9181 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9241 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9301 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9361 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9421 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9541 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9601 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9661 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9721 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     9781 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9841 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9901 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9961 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10021 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10081 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10141 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10201 cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta
    10261 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
    10321 tcataatggg gaaggccatc cagcctcgcg tcgcgataac cctaattcga tagcatatgc
    10381 ttcccgttgg gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac
    10441 ccgggaagca tatgctaccc gtttagggtt caccggtgat gccggccacg atgcgtccgg
    10501 cgtagaggat ctaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10561 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10621 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    10681 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
    10741 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
    10801 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
    10861 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaac
//
