LOCUS       sequence-319607-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..66
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     17..37
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    440..521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(477..496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(532..549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(783..802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
       61 agagctggtt tagtgaaccg tcagatccgc tagggatcct ctagtcagct gacgcgtgct
      121 agcgcggccg caagaagctc tctgctcagc ctgtcacaac ctactgactg ccagggcact
      181 tgggaatggc aaggaaaccg ttaccattac tgagtttagt aatggtaatg gttctcttgc
      241 tatacccaga aaacgtgcca ggaagagaac tcaggaccct gaagcagact actggaaggg
      301 agactccaaa gcttgtcgac gatatctcca gaggatcata atcagccata ccacatttgt
      361 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      421 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      481 tagcatcaca aatttcacaa ataaagcatt tttttcactg ccccgagctt cctcgctcac
      541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      781 ataaagatac caggcgtttc cc
//
