LOCUS       sequence-319628-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His6-Delta-MEK1C218C222.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, 
            Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair 
            SK, Chen J, Davis BG, van der Donk WA
  TITLE     LanCLs add glutathione to dehydroamino acids generated at 
            phosphorylated sites in the proteome.
  JOURNAL   Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001.
  PUBMED    33932340
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001."
COMMENT     Sequence Label: Delta-1-60MEK1C218C222
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             970..987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaactga aagatgacnn tttcgaaaaa atcagtgaac tgggcgccgg taacggcggt
       61 gtggttttta aagtcagcca taaaccgtct ggtctggtga tggcacgtaa actgattcac
      121 ctggaaatca aaccggctat tcgtaaccag attatccgcg aactgcaagt gctgcatgaa
      181 tgcaattctc cgtatattgt tggcttttat ggtgcgttct acagtgacgg cgaaatttcc
      241 atctgtatgg aacacatgga cggcggtagc ctggatcagg ttctgaaaaa agcaggccgt
      301 atcccggaac aaattctggg taaagtctct attgctgtga tcaaaggcct gacgtacctg
      361 cgtgaaaaac ataaaatcat gcaccgcgat gtgaaaccgt caaacatcct ggttaattcg
      421 cgcggtgaaa ttaaactgtg cgactttggc gttagcggtc agctgattga ttgtatggcg
      481 aactgcttcg tcggcacccg tagttatatg tccccggaac gcctgcaggg tacgcattac
      541 tcagtgcaat cggatatctg gtcaatgggc ctgtcgctgg ttgaaatggc cgttggtcgt
      601 tatccgattc cgccgccgga cgccaaagaa ctggaactga tgtttggctc tcaggttgaa
      661 ggtgatgcgg ccgaaacccc gccgcgtccg cgtaccccgg gtcgtccgct gagctcttat
      721 ggtatggaca gccgtccgcc gatggcaatc ttcgaactgc tggattacat tgtgaatgaa
      781 ccgccgccaa aactgccgag cggcgtgttt agcctggaat ttcaggactt cgtcaacaaa
      841 tgtctgatca aaaatccggc agaacgtgct gatctgaaac aactgatggt ccacgctttt
      901 attaaacgct ccgacgcgga agaagtggat ttcgccggtt ggctgtcttc gaccattggc
      961 ctgaatgcgc accatcatca ccaccattga
//
