LOCUS       sequence-319811-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-374b and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir374b-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    473..554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(510..529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(565..582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agggatcctc
       61 tagtcagctg acgcgtgcta gcgcggccgc agcacgtgac aaaaacaaca gcagaccctg
      121 gtgcctggga ggacttcatg gatccagcag caacctggag tggtgctcct ctgaagaaat
      181 cctactcgga tggatataat acaacctgct aagtgtccta gcacttagca ggttgtatta
      241 tcattgtccg tgtctatggc tctcgtctac cagactttaa attccttaag ggcaaggaca
      301 gtgccttact catctttgta ttcacagtgc ctaatccggt gaagcttgtc gacgatatct
      361 ccagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      421 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      541 atttttttca ctgccccgag cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      661 taacgcagga aagaacat
//
