LOCUS       sequence-319819-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144[309 nt]~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144[309 nt]~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(288..306)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    783..864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(820..839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagggat cctctagtca gctgacgcgt
       61 gctagcgcgg ccgcgttctg cccccagccc ctcacagtgc ttttcaagcc atgcttcctg
      121 tgcccccagt ggggccctgg ctgggatatc atcatatact gtaagtttgc gatgagacac
      181 tacagtatag atgatgtact agtccgggca cccccagctc tggagcctga caaggaggac
      241 aggagagatg ctgcaagccc aagggtaccc tggaagatgg aaccgctgga gagcaactgc
      301 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
      361 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
      421 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgac ctgcaggaag
      481 ctctctgctc agcctgtcac aacctactga ctgccagggc acttgggaat ggcaaggaaa
      541 ccgttaccat tactgagttt agtaatggta atggttctct tgctataccc agaaaacgtg
      601 ccaggaagag aactcaggac cctgaagcag actactggaa gggagactcc aaagcttgtc
      661 gacgatatct ccagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      721 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      781 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      841 caaataaagc atttttttca ctgccccgag cttcctcgc
//
