LOCUS       sequence-319856-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144[909 nt]~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144[909 nt]~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(281..299)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagg gatcctctag tcagctgacg cgtgctagcg
       61 cggccgcgtt ctgcccccag cccctcacag tgcttttcaa gccatgcttc ctgtgccccc
      121 agtggggccc tggctgggat atcatcatat actgtaagtt tgcgatgaga cactacagta
      181 tagatgatgt actagtccgg gcacccccag ctctggagcc tgacaaggag gacaggagag
      241 atgctgcaag cccaagggta ccctggaaga tggaaccgct ggagagcaac tgcataaggc
      301 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      361 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
      421 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
      481 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
      541 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
      601 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
      661 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
      721 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
      781 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
      841 cctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
      901 tcaaatcatt c
//
